Gene description for Ankfy1
Gene name ankyrin repeat and FYVE domain containing 1
Gene symbol Ankfy1
Other names/aliases Ankhzn
ZFYVE14
mKIAA1255
Species Mus musculus
 Database cross references - Ankfy1
ExoCarta ExoCarta_11736
Vesiclepedia VP_11736
Entrez Gene 11736
UniProt Q810B6  
 Ankfy1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ankfy1
Molecular Function
    small GTPase binding GO:0031267 IBA
    small GTPase binding GO:0031267 IEA
    small GTPase binding GO:0031267 ISO
    metal ion binding GO:0046872 IEA
    phosphatidylinositol phosphate binding GO:1901981 IEA
    phosphatidylinositol phosphate binding GO:1901981 ISO
Biological Process
    endocytosis GO:0006897 NAS
    endosomal transport GO:0016197 ISO
    endosomal vesicle fusion GO:0034058 IEA
    endosomal vesicle fusion GO:0034058 ISO
    retrograde transport, endosome to Golgi GO:0042147 IBA
    retrograde transport, endosome to Golgi GO:0042147 IEA
    retrograde transport, endosome to Golgi GO:0042147 ISO
    positive regulation of pinocytosis GO:0048549 IEA
    positive regulation of pinocytosis GO:0048549 ISO
    Golgi to lysosome transport GO:0090160 IBA
    Golgi to lysosome transport GO:0090160 IEA
    Golgi to lysosome transport GO:0090160 ISO
Subcellular Localization
    endosome GO:0005768 ISO
    early endosome GO:0005769 IEA
    early endosome GO:0005769 ISO
    cytosol GO:0005829 IEA
    endosome membrane GO:0010008 IBA
    endosome membrane GO:0010008 IDA
    membrane GO:0016020 ISO
    retromer complex GO:0030904 IEA
    retromer complex GO:0030904 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    macropinosome GO:0044354 IEA
    macropinosome GO:0044354 ISO
 Experiment description of studies that identified Ankfy1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ankfy1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dcaf5  
Co-fractionation Mus musculus
2 Aldh7a1  
Co-fractionation Mus musculus
3 Snx6 72183
Co-fractionation Mus musculus
4 Fxr1 14359
Affinity Capture-MS Mus musculus
5 Dcaf11  
Co-fractionation Mus musculus
6 Vps26a 30930
Co-fractionation Mus musculus
7 Dda1  
Co-fractionation Mus musculus
8 Aldh9a1 56752
Co-fractionation Mus musculus
9 Vps35 65114
Co-fractionation Mus musculus
10 COX6B1 1340
Affinity Capture-MS Homo sapiens
11 Snx2 67804
Co-fractionation Mus musculus
12 Snx1  
Co-fractionation Mus musculus
13 Snx3 54198
Co-fractionation Mus musculus
14 RPUSD1  
Affinity Capture-MS Homo sapiens
15 ANKRD52 283373
Affinity Capture-MS Homo sapiens
16 Adsl 11564
Co-fractionation Mus musculus
17 Wars 22375
Co-fractionation Mus musculus
18 Krt77 406220
Co-fractionation Mus musculus
19 Dsc1  
Co-fractionation Mus musculus
20 Sstr3  
Proximity Label-MS Mus musculus
21 ABCA2 20
Affinity Capture-MS Homo sapiens
22 Eed  
Affinity Capture-MS Mus musculus
23 ANXA2 302
Affinity Capture-MS Homo sapiens
24 Lta4h 16993
Co-fractionation Mus musculus
25 A230046K03Rik  
Co-fractionation Mus musculus
26 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here