Gene description for Ankfy1
Gene name ankyrin repeat and FYVE domain containing 1
Gene symbol Ankfy1
Other names/aliases Ankhzn
ZFYVE14
mKIAA1255
Species Mus musculus
 Database cross references - Ankfy1
ExoCarta ExoCarta_11736
Vesiclepedia VP_11736
Entrez Gene 11736
UniProt Q810B6  
 Ankfy1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ankfy1
Molecular Function
    phosphatidylinositol phosphate binding GO:1901981 ISO
    metal ion binding GO:0046872 IEA
    Rab GTPase binding GO:0017137 ISO
Biological Process
    retrograde transport, endosome to Golgi GO:0042147 ISO
    endosomal vesicle fusion GO:0034058 ISO
    endosome localization GO:0032439 ISO
    endocytosis GO:0006897 NAS
    Golgi to lysosome transport GO:0090160 ISO
    positive regulation of pinocytosis GO:0048549 ISO
Subcellular Localization
    early endosome GO:0005769 ISO
    cytoplasm GO:0005737 IEA
    cytoplasmic vesicle GO:0031410 IEA
    membrane GO:0016020 ISO
    extracellular exosome GO:0070062 ISO
    retromer complex GO:0030904 ISO
    endosome GO:0005768 IEA
    lysosomal membrane GO:0005765 ISO
    endosome membrane GO:0010008 IDA
    macropinosome GO:0044354 ISO
 Experiment description of studies that identified Ankfy1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ankfy1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ankfy1 is involved
No pathways found





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