Gene description for Vps35
Gene name vacuolar protein sorting 35
Gene symbol Vps35
Other names/aliases AI647796
Mem3
Species Mus musculus
 Database cross references - Vps35
ExoCarta ExoCarta_65114
Vesiclepedia VP_65114
Entrez Gene 65114
UniProt Q9EQH3  
 Vps35 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Vps35
Molecular Function
    protein binding GO:0005515 IPI
    D1 dopamine receptor binding GO:0031748 IPI
    D1 dopamine receptor binding GO:0031748 ISO
Biological Process
    vacuolar protein processing GO:0006624 ISA
    intracellular protein transport GO:0006886 IBA
    lysosome organization GO:0007040 IMP
    positive regulation of gene expression GO:0010628 IMP
    positive regulation of gene expression GO:0010628 ISO
    negative regulation of gene expression GO:0010629 IMP
    regulation of mitochondrion organization GO:0010821 ISO
    regulation of protein stability GO:0031647 ISO
    protein destabilization GO:0031648 IMP
    endocytic recycling GO:0032456 ISO
    endocytic recycling GO:0032456 ISS
    negative regulation of protein homooligomerization GO:0032463 IMP
    protein localization to organelle GO:0033365 IMP
    protein localization to endosome GO:0036010 ISO
    protein localization to endosome GO:0036010 ISS
    retrograde transport, endosome to Golgi GO:0042147 IBA
    retrograde transport, endosome to Golgi GO:0042147 IC
    retrograde transport, endosome to Golgi GO:0042147 ISO
    retrograde transport, endosome to Golgi GO:0042147 ISS
    mitochondrial fragmentation involved in apoptotic process GO:0043653 IEA
    mitochondrial fragmentation involved in apoptotic process GO:0043653 ISO
    transcytosis GO:0045056 ISO
    transcytosis GO:0045056 ISS
    positive regulation of protein catabolic process GO:0045732 IEA
    positive regulation of protein catabolic process GO:0045732 ISO
    negative regulation of inflammatory response GO:0050728 IEA
    negative regulation of inflammatory response GO:0050728 ISO
    modulation of chemical synaptic transmission GO:0050804 IDA
    modulation of chemical synaptic transmission GO:0050804 IEP
    modulation of chemical synaptic transmission GO:0050804 IMP
    voluntary musculoskeletal movement GO:0050882 IMP
    regulation of protein metabolic process GO:0051246 ISO
    positive regulation of dopamine receptor signaling pathway GO:0060161 IEA
    positive regulation of dopamine receptor signaling pathway GO:0060161 ISO
    positive regulation of Wnt protein secretion GO:0061357 IMP
    regulation of synapse maturation GO:0090128 IDA
    regulation of synapse maturation GO:0090128 IMP
    positive regulation of mitochondrial fission GO:0090141 IEA
    positive regulation of mitochondrial fission GO:0090141 ISO
    positive regulation of canonical Wnt signaling pathway GO:0090263 IGI
    positive regulation of locomotion involved in locomotory behavior GO:0090326 IMP
    neurotransmitter receptor transport, endosome to postsynaptic membrane GO:0098887 IDA
    neurotransmitter receptor transport, endosome to postsynaptic membrane GO:0098887 IMP
    vesicle-mediated transport in synapse GO:0099003 IDA
    vesicle-mediated transport in synapse GO:0099003 IMP
    mitochondrion to lysosome vesicle-mediated transport GO:0099074 IEA
    mitochondrion to lysosome vesicle-mediated transport GO:0099074 ISO
    neurotransmitter receptor transport, endosome to plasma membrane GO:0099639 IMP
    neurotransmitter receptor transport, endosome to plasma membrane GO:0099639 ISO
    regulation of postsynapse assembly GO:0150052 IDA
    regulation of postsynapse assembly GO:0150052 IMP
    negative regulation of late endosome to lysosome transport GO:1902823 IEA
    negative regulation of late endosome to lysosome transport GO:1902823 ISO
    regulation of dendritic spine maintenance GO:1902950 IEA
    regulation of dendritic spine maintenance GO:1902950 ISO
    positive regulation of dopamine biosynthetic process GO:1903181 IMP
    negative regulation of protein localization GO:1903828 IMP
    positive regulation of protein localization to cell periphery GO:1904377 IEA
    positive regulation of protein localization to cell periphery GO:1904377 ISO
    negative regulation of lysosomal protein catabolic process GO:1905166 IMP
    regulation of terminal button organization GO:2000331 IEA
    regulation of terminal button organization GO:2000331 ISO
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 IMP
    mitochondrion GO:0005739 IEA
    mitochondrion GO:0005739 ISO
    lysosome GO:0005764 IEA
    lysosome GO:0005764 ISO
    endosome GO:0005768 IDA
    endosome GO:0005768 ISO
    endosome GO:0005768 ISS
    early endosome GO:0005769 IEA
    early endosome GO:0005769 ISO
    late endosome GO:0005770 IBA
    late endosome GO:0005770 IEA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    endosome membrane GO:0010008 IEA
    endosome membrane GO:0010008 ISO
    postsynaptic density GO:0014069 IDA
    membrane GO:0016020 ISA
    retromer complex GO:0030904 IBA
    retromer complex GO:0030904 IDA
    retromer complex GO:0030904 ISO
    retromer, cargo-selective complex GO:0030906 IEA
    retromer, cargo-selective complex GO:0030906 ISO
    neuron projection GO:0043005 IMP
    neuronal cell body GO:0043025 IMP
    synapse GO:0045202 IDA
    perinuclear region of cytoplasm GO:0048471 IMP
    tubular endosome GO:0097422 ISO
    tubular endosome GO:0097422 ISS
    dopaminergic synapse GO:0098691 IDA
    dopaminergic synapse GO:0098691 IEP
    dopaminergic synapse GO:0098691 IMP
    presynapse GO:0098793 IDA
    postsynapse GO:0098794 IDA
    postsynapse GO:0098794 IEP
    postsynapse GO:0098794 IMP
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IEP
    glutamatergic synapse GO:0098978 IMP
    mitochondrion-derived vesicle GO:0099073 IEA
    mitochondrion-derived vesicle GO:0099073 ISO
 Experiment description of studies that identified Vps35 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Vps35
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Snx1  
Co-fractionation Mus musculus
2 Snx2 67804
Co-fractionation Mus musculus
3 Tbc1d5  
Co-fractionation Mus musculus
4 Atxn1  
Affinity Capture-MS Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Ankfy1 11736
Co-fractionation Mus musculus
7 Psap 19156
Co-fractionation Mus musculus
8 Plxdc2 67448
Co-fractionation Mus musculus
9 Vps26b  
Co-fractionation Mus musculus
10 Mapt  
Affinity Capture-MS Mus musculus
11 Snx6 72183
Co-fractionation Mus musculus
12 Rab7 19349
Co-fractionation Mus musculus
13 Unk  
Affinity Capture-RNA Mus musculus
14 Atg12  
Negative Genetic Mus musculus
15 Vps29 56433
Co-fractionation Mus musculus
16 Fancd2  
Affinity Capture-MS Mus musculus
17 Kctd13  
Affinity Capture-MS Mus musculus
18 Vps26a 30930
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Vps35 is involved
PathwayEvidenceSource
Signal Transduction IEA Reactome
Signaling by WNT IEA Reactome
WNT ligand biogenesis and trafficking IEA Reactome





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