Gene description for Psap
Gene name prosaposin
Gene symbol Psap
Other names/aliases AI037048
SGP-1
Species Mus musculus
 Database cross references - Psap
ExoCarta ExoCarta_19156
Vesiclepedia VP_19156
Entrez Gene 19156
UniProt Q61207  
 Psap identified in sEVs derived from the following tissue/cell type
Faeces 33431899    
Faeces 33431899    
Fibroblasts 23260141    
 Gene ontology annotations for Psap
Molecular Function
    G protein-coupled receptor binding GO:0001664 IPI
    protease binding GO:0002020 ISO
    protein binding GO:0005515 IPI
    phospholipid binding GO:0005543 ISO
    lipid antigen binding GO:0030882 IDA
    identical protein binding GO:0042802 IPI
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISO
    scaffold protein binding GO:0097110 ISO
    ganglioside GM1 binding GO:1905573 ISO
    ganglioside GM2 binding GO:1905574 ISO
    ganglioside GM3 binding GO:1905575 ISO
    ganglioside GT1b binding GO:1905576 ISO
    ganglioside GP1c binding GO:1905577 ISO
Biological Process
    NK T cell differentiation GO:0001865 IMP
    corneocyte development GO:0003335 IMP
    membrane lipid metabolic process GO:0006643 IDA
    glycolipid metabolic process GO:0006664 IMP
    sphingolipid metabolic process GO:0006665 IMP
    ceramide metabolic process GO:0006672 IMP
    glucosylceramide metabolic process GO:0006678 IMP
    galactosylceramide catabolic process GO:0006683 IMP
    intracellular protein transport GO:0006886 IMP
    lysosomal transport GO:0007041 ISO
    lysosomal transport GO:0007041 ISS
    adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway GO:0007193 IBA
    adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway GO:0007193 IDA
    sensory perception of sound GO:0007605 IMP
    protein secretion GO:0009306 IMP
    gene expression GO:0010467 IDA
    gene expression GO:0010467 IMP
    regulation of lipid metabolic process GO:0019216 IBA
    regulation of lipid metabolic process GO:0019216 IMP
    antigen processing and presentation GO:0019882 IMP
    cerebellar Purkinje cell differentiation GO:0021702 IMP
    cellular response to reactive oxygen species GO:0034614 IDA
    myelination GO:0042552 IMP
    regulation of MAPK cascade GO:0043408 IMP
    positive regulation of MAPK cascade GO:0043410 IDA
    developmental growth GO:0048589 IMP
    nervous system process GO:0050877 IMP
    neuromuscular process controlling balance GO:0050885 IMP
    positive regulation of hydrolase activity GO:0051345 IMP
    micturition GO:0060073 IMP
    prostate gland growth GO:0060736 IBA
    prostate gland growth GO:0060736 IMP
    epithelial cell differentiation involved in prostate gland development GO:0060742 IBA
    epithelial cell differentiation involved in prostate gland development GO:0060742 IMP
    inclusion body assembly GO:0070841 IMP
    renal system development GO:0072001 IMP
    cochlea development GO:0090102 IMP
    walking behavior GO:0090659 IMP
    cornified envelope assembly GO:1903575 IMP
    positive regulation of beta-galactosidase activity GO:1903771 ISO
    lysosomal protein catabolic process GO:1905146 IMP
    ganglioside GM1 transport to membrane GO:1905572 ISO
Subcellular Localization
    extracellular region GO:0005576 IDA
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 ISO
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HDA
    lysosome GO:0005764 IBA
    lysosome GO:0005764 IDA
    lysosome GO:0005764 ISO
    lysosome GO:0005764 ISS
    late endosome GO:0005770 ISO
    late endosome GO:0005770 ISS
    membrane GO:0016020 IEA
    aggresome GO:0016235 IDA
    protein-containing complex GO:0032991 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Psap in sEVs
1
Experiment ID 303
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
2
Experiment ID 304
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
3
Experiment ID 210
MISEV standards
EM
Biophysical techniques
CD81|FLOT1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Psap
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Vps35 65114
Co-fractionation Mus musculus
2 Sort1  
Affinity Capture-Western Mus musculus
3 Sema4a  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
View the network image/svg+xml



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