Gene description for Adsl
Gene name adenylosuccinate lyase
Gene symbol Adsl
Other names/aliases Adl
Asl
Species Mus musculus
 Database cross references - Adsl
ExoCarta ExoCarta_11564
Vesiclepedia VP_11564
Entrez Gene 11564
UniProt P54822  
 Adsl identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Adsl
Molecular Function
    N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity GO:0004018 IBA
    N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity GO:0004018 IDA
    N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity GO:0004018 ISA
    N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity GO:0004018 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity GO:0070626 IBA
    (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity GO:0070626 IMP
    (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity GO:0070626 ISO
Biological Process
    response to hypoxia GO:0001666 IEA
    AMP biosynthetic process GO:0006167 ISA
    AMP biosynthetic process GO:0006167 ISO
    GMP biosynthetic process GO:0006177 IMP
    'de novo' IMP biosynthetic process GO:0006189 IEA
    'de novo' IMP biosynthetic process GO:0006189 IMP
    response to nutrient GO:0007584 IEA
    response to nutrient GO:0007584 ISO
    aerobic respiration GO:0009060 IEA
    aerobic respiration GO:0009060 ISO
    response to muscle activity GO:0014850 IEA
    response to starvation GO:0042594 IEA
    'de novo' AMP biosynthetic process GO:0044208 IBA
    'de novo' AMP biosynthetic process GO:0044208 IDA
    'de novo' AMP biosynthetic process GO:0044208 IEA
    'de novo' AMP biosynthetic process GO:0044208 IMP
    AMP salvage GO:0044209 IDA
    AMP salvage GO:0044209 TAS
    'de novo' XMP biosynthetic process GO:0097294 IMP
Subcellular Localization
    mitochondrion GO:0005739 HDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    protein-containing complex GO:0032991 IEA
    protein-containing complex GO:0032991 ISO
 Experiment description of studies that identified Adsl in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Adsl
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Wdr4  
Co-fractionation Mus musculus
2 Atxn1  
Affinity Capture-MS Mus musculus
3 Foxp3  
Affinity Capture-MS Mus musculus
4 Twf1 19230
Co-fractionation Mus musculus
5 Ankfy1 11736
Co-fractionation Mus musculus
6 Fancd2  
Affinity Capture-MS Mus musculus
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