Gene description for Twf1
Gene name twinfilin, actin-binding protein, homolog 1 (Drosophila)
Gene symbol Twf1
Other names/aliases A6
Ptk9
twinfilin
Species Mus musculus
 Database cross references - Twf1
ExoCarta ExoCarta_19230
Vesiclepedia VP_19230
Entrez Gene 19230
UniProt Q91YR1  
 Twf1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Twf1
Molecular Function
    actin binding GO:0003779 ISO
    actin monomer binding GO:0003785 IBA
    actin monomer binding GO:0003785 IDA
    protein tyrosine kinase activity GO:0004713 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IDA
    protein-containing complex binding GO:0044877 IPI
    actin filament binding GO:0051015 IBA
Biological Process
    regulation of lamellipodium assembly GO:0010591 IBA
    positive regulation of cardiac muscle hypertrophy GO:0010613 IDA
    positive regulation of neuron projection development GO:0010976 IBA
    actin filament depolymerization GO:0030042 IBA
    negative regulation of actin filament polymerization GO:0030837 IDA
    sequestering of actin monomers GO:0042989 IBA
    sequestering of actin monomers GO:0042989 IDA
    barbed-end actin filament capping GO:0051016 IBA
    barbed-end actin filament capping GO:0051016 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    actin filament GO:0005884 IBA
    cell-cell junction GO:0005911 IDA
    actin cytoskeleton GO:0015629 IDA
    myofibril GO:0030016 IBA
    myofibril GO:0030016 IDA
    filopodium GO:0030175 IDA
    ruffle membrane GO:0032587 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified Twf1 in sEVs
1
Experiment ID 907
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Twf1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Wars 22375
Co-fractionation Mus musculus
2 Bmpr2 12168
Co-fractionation Mus musculus
3 Usp5 22225
Co-fractionation Mus musculus
4 Atxn1  
Affinity Capture-MS Mus musculus
5 Kctd13  
Affinity Capture-MS Mus musculus
6 Mycbp2 105689
Affinity Capture-MS Mus musculus
7 Adsl 11564
Co-fractionation Mus musculus
8 BMPR2 659
Affinity Capture-MS Homo sapiens
9 Atg16l1  
Affinity Capture-MS Mus musculus
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