Gene description for Twf1
Gene name twinfilin, actin-binding protein, homolog 1 (Drosophila)
Gene symbol Twf1
Other names/aliases A6
Ptk9
twinfilin
Species Mus musculus
 Database cross references - Twf1
ExoCarta ExoCarta_19230
Vesiclepedia VP_19230
Entrez Gene 19230
UniProt Q91YR1  
 Twf1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Twf1
Molecular Function
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IDA
    protein complex binding GO:0032403 IPI
    protein tyrosine kinase activity GO:0004713 IDA
    ATP binding GO:0005524 ISO
    actin monomer binding GO:0003785 IDA
    actin binding GO:0003779 IEA
Biological Process
    regulation of actin phosphorylation GO:0043538 ISO
    sequestering of actin monomers GO:0042989 IDA
    negative regulation of actin filament polymerization GO:0030837 IDA
    peptidyl-tyrosine phosphorylation GO:0018108 IDA
    barbed-end actin filament capping GO:0051016 IDA
Subcellular Localization
    cytoskeleton GO:0005856 IEA
    intracellular GO:0005622 IEA
    cytoplasm GO:0005737 ISO
    actin cytoskeleton GO:0015629 IDA
    ruffle membrane GO:0032587 IDA
    cell-cell junction GO:0005911 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    myofibril GO:0030016 IDA
    filopodium GO:0030175 IDA
    focal adhesion GO:0005925 ISO
 Experiment description of studies that identified Twf1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Twf1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BMPR2 659
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Twf1 is involved
No pathways found





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