Gene description for Usp5
Gene name ubiquitin specific peptidase 5 (isopeptidase T)
Gene symbol Usp5
Other names/aliases AA407472
ISOT
ISOT-1
Ucht
Species Mus musculus
 Database cross references - Usp5
ExoCarta ExoCarta_22225
Entrez Gene 22225
UniProt P56399  
 Usp5 identified in exosomes derived from the following tissue/cell type
Neural stem cells 25242146    
Pancreatic cells 19351151    
 Gene ontology annotations for Usp5
Molecular Function
    cysteine-type peptidase activity GO:0008234 IEA
    zinc ion binding GO:0008270 IEA
    peptidase activity GO:0008233 IEA
    hydrolase activity GO:0016787 IEA
    ubiquitinyl hydrolase activity GO:0036459 IEA
    omega peptidase activity GO:0008242 IEA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    cysteine-type endopeptidase activity GO:0004197 IBA
    ubiquitin-specific protease activity GO:0004843 IBA
Biological Process
    regulation of proteasomal protein catabolic process GO:0061136 IBA
    protein K48-linked deubiquitination GO:0071108 ISO
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 IEA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    protein deubiquitination GO:0016579 IBA
    proteolysis GO:0006508 IEA
Subcellular Localization
    lysosome GO:0005764 IBA
    cellular_component GO:0005575 ND
 Experiment description of studies that identified Usp5 in exosomes
1
Experiment ID 263
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Usp5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Usp5 is involved
No pathways found





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