Gene description for Casp1
Gene name caspase 1
Gene symbol Casp1
Other names/aliases ICE
Il1bc
Species Mus musculus
 Database cross references - Casp1
ExoCarta ExoCarta_12362
Vesiclepedia VP_12362
Entrez Gene 12362
UniProt P29452  
 Casp1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Casp1
Molecular Function
    hydrolase activity GO:0016787 IEA
    cysteine-type peptidase activity GO:0008234 IEA
    scaffold protein binding GO:0097110 ISO
    cysteine-type endopeptidase activity GO:0004197 IEA
    peptidase activity GO:0008233 IDA
    protein binding GO:0005515 IPI
    endopeptidase activity GO:0004175 ISO
Biological Process
    membrane hyperpolarization GO:0060081 IMP
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 IDA
    apoptotic process GO:0006915 IBA
    regulation of autophagy GO:0010506 IMP
    positive regulation of interleukin-1 alpha secretion GO:0050717 IMP
    programmed necrotic cell death GO:0097300 IDA
    response to drug GO:0042493 ISO
    protein processing GO:0016485 IMP
    response to organic cyclic compound GO:0014070 ISO
    cellular response to organic substance GO:0071310 ISO
    response to ATP GO:0033198 IMP
    proteolysis GO:0006508 ISO
    positive regulation of circadian sleep/wake cycle, non-REM sleep GO:0046010 ISO
    response to lipopolysaccharide GO:0032496 ISO
    regulation of apoptotic process GO:0042981 IEA
    myoblast fusion GO:0007520 ISO
    response to bacterium GO:0009617 IMP
    toxin transport GO:1901998 IMP
    mitochondrial depolarization GO:0051882 IMP
    regulation of inflammatory response GO:0050727 IMP
    microglial cell activation GO:0001774 ISO
    positive regulation of cytokine secretion GO:0050715 ISO
    interleukin-1 beta production GO:0032611 IGI
    positive regulation of interleukin-1 beta secretion GO:0050718 ISO
    response to hypoxia GO:0001666 ISO
    positive regulation of apoptotic process GO:0043065 ISO
    memory GO:0007613 ISO
    pyroptosis GO:0070269 IMP
Subcellular Localization
    protein complex GO:0043234 ISO
    NLRP3 inflammasome complex GO:0072559 ISO
    nucleus GO:0005634 ISO
    neuron projection GO:0043005 ISO
    AIM2 inflammasome complex GO:0097169 ISO
    cytoplasm GO:0005737 TAS
    extracellular region GO:0005576 IDA
    IPAF inflammasome complex GO:0072557 IDA
    mitochondrion GO:0005739 IMP
    NLRP1 inflammasome complex GO:0072558 ISO
 Experiment description of studies that identified Casp1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Casp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Casp1 is involved
PathwayEvidenceSource
Interleukin-1 processing IEA Reactome
The AIM2 inflammasome IEA Reactome
The NLRP3 inflammasome IEA Reactome





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