Gene description for Ube2n
Gene name ubiquitin-conjugating enzyme E2N
Gene symbol Ube2n
Other names/aliases 1500026J17Rik
AL022654
BB101821
UBC13
Species Mus musculus
 Database cross references - Ube2n
ExoCarta ExoCarta_93765
Vesiclepedia VP_93765
Entrez Gene 93765
UniProt P61089  
 Ube2n identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Oligodendrocytes 21136642    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ube2n
Molecular Function
    nucleotide binding GO:0000166 IEA
    ligase activity GO:0016874 IEA
    ubiquitin protein ligase activity GO:0061630 IBA
    protein binding GO:0005515 IPI
    ubiquitin binding GO:0043130 ISO
    ubiquitin conjugating enzyme activity GO:0061631 IGI
    ubiquitin protein ligase binding GO:0031625 IBA
    poly(A) RNA binding GO:0044822 ISO
    ATP binding GO:0005524 IEA
    ubiquitin-protein transferase activity GO:0004842 ISO
Biological Process
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 ISO
    regulation of histone ubiquitination GO:0033182 ISO
    postreplication repair GO:0006301 ISO
    positive regulation of DNA repair GO:0045739 ISO
    DNA repair GO:0006281 IEA
    double-strand break repair via homologous recombination GO:0000724 ISO
    protein ubiquitination GO:0016567 ISO
    protein K63-linked ubiquitination GO:0070534 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    histone ubiquitination GO:0016574 ISO
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISS
    cellular response to DNA damage stimulus GO:0006974 IEA
    positive regulation of ubiquitin-protein transferase activity GO:0051443 ISO
    T cell receptor signaling pathway GO:0050852 ISS
    DNA double-strand break processing GO:0000729 ISO
    positive regulation of histone modification GO:0031058 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    UBC13-MMS2 complex GO:0031372 ISO
    ubiquitin ligase complex GO:0000151 ISO
    nucleus GO:0005634 ISO
    UBC13-UEV1A complex GO:0035370 ISO
    extracellular exosome GO:0070062 ISO
    protein complex GO:0043234 ISO
 Experiment description of studies that identified Ube2n in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD81|FLOT1
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 33
MISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
Alix|TSG101
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ube2n
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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