Gene description for Ube2n
Gene name ubiquitin-conjugating enzyme E2N
Gene symbol Ube2n
Other names/aliases 1500026J17Rik
AL022654
BB101821
UBC13
Species Mus musculus
 Database cross references - Ube2n
ExoCarta ExoCarta_93765
Vesiclepedia VP_93765
Entrez Gene 93765
UniProt P61089  
 Ube2n identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Oligodendrocytes 21136642    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ube2n
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 ISO
    ubiquitin-protein transferase activity GO:0004842 ISS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    ubiquitin binding GO:0043130 ISO
    ubiquitin binding GO:0043130 ISS
    ubiquitin conjugating enzyme activity GO:0061631 IDA
    ubiquitin conjugating enzyme activity GO:0061631 IDA
    ubiquitin conjugating enzyme activity GO:0061631 ISO
    ubiquitin conjugating enzyme activity GO:0061631 ISS
    ubiquitin-protein transferase activator activity GO:0097027 ISO
    ubiquitin-protein transferase activator activity GO:0097027 ISS
Biological Process
    double-strand break repair via homologous recombination GO:0000724 ISO
    double-strand break repair via homologous recombination GO:0000724 ISS
    DNA double-strand break processing GO:0000729 ISO
    DNA double-strand break processing GO:0000729 ISS
    postreplication repair GO:0006301 IBA
    postreplication repair GO:0006301 ISO
    postreplication repair GO:0006301 ISS
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    protein monoubiquitination GO:0006513 ISS
    protein ubiquitination GO:0016567 ISO
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 ISO
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 ISS
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IEA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 ISO
    positive regulation of DNA repair GO:0045739 ISO
    positive regulation of DNA repair GO:0045739 ISS
    T cell receptor signaling pathway GO:0050852 ISO
    T cell receptor signaling pathway GO:0050852 ISS
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISO
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISS
    protein K63-linked ubiquitination GO:0070534 IBA
    protein K63-linked ubiquitination GO:0070534 IDA
    protein K63-linked ubiquitination GO:0070534 ISO
    protein K63-linked ubiquitination GO:0070534 ISS
    regulation protein catabolic process at postsynapse GO:0140252 IEA
    antiviral innate immune response GO:0140374 IEA
    antiviral innate immune response GO:0140374 ISO
    positive regulation of protein K63-linked ubiquitination GO:1902523 ISO
    positive regulation of intracellular signal transduction GO:1902533 ISO
    negative regulation of TORC1 signaling GO:1904262 IEA
    negative regulation of TORC1 signaling GO:1904262 ISO
    positive regulation of double-strand break repair GO:2000781 ISO
Subcellular Localization
    ubiquitin ligase complex GO:0000151 ISO
    ubiquitin ligase complex GO:0000151 ISS
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 ISO
    ubiquitin conjugating enzyme complex GO:0031371 ISO
    UBC13-MMS2 complex GO:0031372 ISO
    UBC13-MMS2 complex GO:0031372 ISS
    protein-containing complex GO:0032991 ISO
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified Ube2n in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ube2n
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ubc  
Reconstituted Complex Mus musculus
2 Ube2v2  
Two-hybrid Mus musculus
Two-hybrid Mus musculus
Co-fractionation Mus musculus
3 Zfand5  
Co-fractionation Mus musculus
4 Traf2  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
5 Ube2d1  
Co-fractionation Mus musculus
6 Ube2v1 66589
Two-hybrid Mus musculus
Two-hybrid Mus musculus
Co-fractionation Mus musculus
7 Tnfaip3  
Affinity Capture-Western Mus musculus
8 Dlgap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
9 Trp53  
Affinity Capture-Western Mus musculus
10 Stub1 56424
Co-fractionation Mus musculus
11 Tax1bp1  
Affinity Capture-Western Mus musculus
12 Cd40 21939
Affinity Capture-Western Mus musculus
13 Tlr4  
Affinity Capture-Western Mus musculus
14 Chfr  
Reconstituted Complex Mus musculus
15 Map3k1  
Affinity Capture-Western Mus musculus
16 Traf6  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
17 Rnf41  
Reconstituted Complex Mus musculus
18 Ubxn6  
Co-fractionation Mus musculus
19 Ube2d2a 56550
Co-fractionation Mus musculus
20 Map3k7  
Affinity Capture-Western Mus musculus
21 Uba1 22201
Co-fractionation Mus musculus
22 Ikbkg  
Affinity Capture-Western Mus musculus
23 Rnf8  
Reconstituted Complex Mus musculus
24 Eed  
Affinity Capture-MS Mus musculus
25 UBE2V2 7336
Reconstituted Complex Homo sapiens
26 Atg12  
Positive Genetic Mus musculus
27 Fancd2  
Affinity Capture-MS Mus musculus
28 Tcf3  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Ube2n is involved
PathwayEvidenceSource
activated TAK1 mediates p38 MAPK activation IEA Reactome
Adaptive Immune System IEA Reactome
Aggrephagy IEA Reactome
Antigen processing: Ubiquitination & Proteasome degradation IEA Reactome
Antiviral mechanism by IFN-stimulated genes IEA Reactome
Autophagy IEA Reactome
C-type lectin receptors (CLRs) IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Class I MHC mediated antigen processing & presentation IEA Reactome
CLEC7A (Dectin-1) signaling IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
DNA Double Strand Break Response IEA Reactome
DNA Double-Strand Break Repair IEA Reactome
DNA Repair IEA Reactome
Downstream TCR signaling IEA Reactome
E3 ubiquitin ligases ubiquitinate target proteins IEA Reactome
Fc epsilon receptor (FCERI) signaling IEA Reactome
FCERI mediated NF-kB activation IEA Reactome
Formation of Incision Complex in GG-NER IEA Reactome
G2/M Checkpoints IEA Reactome
G2/M DNA damage checkpoint IEA Reactome
Global Genome Nucleotide Excision Repair (GG-NER) IEA Reactome
HDR through Homologous Recombination (HRR) or Single Strand Annealing (SSA) IEA Reactome
Homology Directed Repair IEA Reactome
IKK complex recruitment mediated by RIP1 IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Interferon Signaling IEA Reactome
Interleukin-1 family signaling IEA Reactome
Interleukin-1 signaling IEA Reactome
Interleukin-17 signaling IEA Reactome
IRAK1 recruits IKK complex IEA Reactome
IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation IEA Reactome
ISG15 antiviral mechanism IEA Reactome
JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 IEA Reactome
Macroautophagy IEA Reactome
MAP kinase activation IEA Reactome
Metabolism of proteins IEA Reactome
Mitophagy IEA Reactome
MyD88 cascade initiated on plasma membrane IEA Reactome
MyD88 dependent cascade initiated on endosome IEA Reactome
MyD88-independent TLR4 cascade IEA Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane IEA Reactome
NOD1/2 Signaling Pathway IEA Reactome
Nonhomologous End-Joining (NHEJ) IEA Reactome
Nucleotide Excision Repair IEA Reactome
Nucleotide-binding domain, leucine rich repeat containing receptor (NLR) signaling pathways IEA Reactome
PINK1-PRKN Mediated Mitophagy IEA Reactome
Post-translational protein modification IEA Reactome
Processing of DNA double-strand break ends IEA Reactome
Protein ubiquitination IEA Reactome
Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks IEA Reactome
Selective autophagy IEA Reactome
Signaling by Interleukins IEA Reactome
TAK1-dependent IKK and NF-kappa-B activation IEA Reactome
TCR signaling IEA Reactome
Toll Like Receptor 10 (TLR10) Cascade IEA Reactome
Toll Like Receptor 2 (TLR2) Cascade IEA Reactome
Toll Like Receptor 3 (TLR3) Cascade IEA Reactome
Toll Like Receptor 4 (TLR4) Cascade IEA Reactome
Toll Like Receptor 5 (TLR5) Cascade IEA Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade IEA Reactome
Toll Like Receptor 9 (TLR9) Cascade IEA Reactome
Toll Like Receptor TLR1:TLR2 Cascade IEA Reactome
Toll Like Receptor TLR6:TLR2 Cascade IEA Reactome
Toll-like Receptor Cascades IEA Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation IEA Reactome
TRIF (TICAM1)-mediated TLR4 signaling IEA Reactome





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