Gene description for Ube2n
Gene name ubiquitin-conjugating enzyme E2N
Gene symbol Ube2n
Other names/aliases 1500026J17Rik
AL022654
BB101821
UBC13
Species Mus musculus
 Database cross references - Ube2n
ExoCarta ExoCarta_93765
Entrez Gene 93765
UniProt P61089  
 Ube2n identified in exosomes derived from the following tissue/cell type
Fibroblasts 23260141    
Oligodendrocytes 21136642    
 Gene ontology annotations for Ube2n
Molecular Function
    nucleotide binding GO:0000166 IEA
    ligase activity GO:0016874 IEA
    ubiquitin protein ligase activity GO:0061630 IBA
    protein binding GO:0005515 IPI
    ubiquitin binding GO:0043130 ISO
    ubiquitin conjugating enzyme activity GO:0061631 IGI
    ubiquitin protein ligase binding GO:0031625 IBA
    poly(A) RNA binding GO:0044822 ISO
    ATP binding GO:0005524 IEA
    ubiquitin-protein transferase activity GO:0004842 ISO
Biological Process
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 ISO
    regulation of histone ubiquitination GO:0033182 ISO
    postreplication repair GO:0006301 ISO
    positive regulation of DNA repair GO:0045739 ISO
    DNA repair GO:0006281 IEA
    double-strand break repair via homologous recombination GO:0000724 ISO
    protein ubiquitination GO:0016567 ISO
    protein K63-linked ubiquitination GO:0070534 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    histone ubiquitination GO:0016574 ISO
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISS
    cellular response to DNA damage stimulus GO:0006974 IEA
    positive regulation of ubiquitin-protein transferase activity GO:0051443 ISO
    T cell receptor signaling pathway GO:0050852 ISS
    DNA double-strand break processing GO:0000729 ISO
    positive regulation of histone modification GO:0031058 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    UBC13-MMS2 complex GO:0031372 ISO
    ubiquitin ligase complex GO:0000151 ISO
    nucleus GO:0005634 ISO
    UBC13-UEV1A complex GO:0035370 ISO
    extracellular exosome GO:0070062 ISO
    protein complex GO:0043234 ISO
 Experiment description of studies that identified Ube2n in exosomes
1
Experiment ID 210
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 33
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Ube2n
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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