Gene description for Actn1
Gene name actinin, alpha 1
Gene symbol Actn1
Other names/aliases 3110023F10Rik
Actn1a
Species Mus musculus
 Database cross references - Actn1
ExoCarta ExoCarta_109711
Entrez Gene 109711
UniProt Q7TPR4  
 Actn1 identified in exosomes derived from the following tissue/cell type
Fibroblasts 23260141    
Pancreatic cells 19351151    
 Gene ontology annotations for Actn1
Molecular Function
    integrin binding GO:0005178 ISO
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    actin filament binding GO:0051015 IDA
    calcium ion binding GO:0005509 IEA
    protein homodimerization activity GO:0042803 TAS
    ion channel binding GO:0044325 ISO
    vinculin binding GO:0017166 ISO
    actin binding GO:0003779 IEA
    double-stranded RNA binding GO:0003725 ISO
    protein domain specific binding GO:0019904 ISO
    ligand-dependent nuclear receptor transcription coactivator activity GO:0030374 ISO
Biological Process
    actin filament bundle assembly GO:0051017 IDA
    cortical cytoskeleton organization GO:0030865 TAS
    negative regulation of cellular component movement GO:0051271 ISO
    actin crosslink formation GO:0051764 IEA
    regulation of nucleic acid-templated transcription GO:1903506 ISO
    focal adhesion assembly GO:0048041 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    cytoskeleton GO:0005856 IEA
    fascia adherens GO:0005916 IDA
    dendritic spine GO:0043197 ISO
    extracellular exosome GO:0070062 ISO
    focal adhesion GO:0005925 TAS
    cytoplasm GO:0005737 ISO
    cortical cytoskeleton GO:0030863 TAS
    membrane GO:0016020 IEA
    dense core granule membrane GO:0032127 TAS
    cell projection GO:0042995 ISO
    cortical actin cytoskeleton GO:0030864 ISO
    Z disc GO:0030018 IDA
    cell junction GO:0030054 IEA
    plasma membrane GO:0005886 IEA
    brush border GO:0005903 IDA
    stress fiber GO:0001725 TAS
    extracellular space GO:0005615 ISO
    secretory granule GO:0030141 NAS
 Experiment description of studies that identified Actn1 in exosomes
1
Experiment ID 210
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD81|FLOT1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Actn1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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