Gene description for Hmgcs1
Gene name 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1
Gene symbol Hmgcs1
Other names/aliases B130032C06Rik
Species Mus musculus
 Database cross references - Hmgcs1
ExoCarta ExoCarta_208715
Vesiclepedia VP_208715
Entrez Gene 208715
UniProt Q8JZK9  
 Hmgcs1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hmgcs1
Molecular Function
    hydroxymethylglutaryl-CoA synthase activity GO:0004421 IBA
    hydroxymethylglutaryl-CoA synthase activity GO:0004421 IEA
    hydroxymethylglutaryl-CoA synthase activity GO:0004421 ISO
    isomerase activity GO:0016853 ISO
    small molecule binding GO:0036094 ISO
    protein homodimerization activity GO:0042803 ISO
    protein homodimerization activity GO:0042803 ISS
    organic acid binding GO:0043177 ISO
Biological Process
    acetyl-CoA metabolic process GO:0006084 IBA
    acetyl-CoA metabolic process GO:0006084 IEA
    cholesterol biosynthetic process GO:0006695 IEA
    farnesyl diphosphate biosynthetic process, mevalonate pathway GO:0010142 IBA
    farnesyl diphosphate biosynthetic process, mevalonate pathway GO:0010142 IEA
    response to purine-containing compound GO:0014074 ISO
    cellular response to low-density lipoprotein particle stimulus GO:0071404 ISO
Subcellular Localization
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified Hmgcs1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hmgcs1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HSD17B10 3028
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Hmgcs1 is involved
PathwayEvidenceSource
Cholesterol biosynthesis IEA Reactome
Metabolism IEA Reactome
Metabolism of lipids IEA Reactome
Metabolism of steroids IEA Reactome





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