Gene description for Asl
Gene name argininosuccinate lyase
Gene symbol Asl
Other names/aliases 2510006M18Rik
Species Mus musculus
 Database cross references - Asl
ExoCarta ExoCarta_109900
Vesiclepedia VP_109900
Entrez Gene 109900
UniProt Q91YI0  
 Asl identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Asl
Molecular Function
    argininosuccinate lyase activity GO:0004056 IBA
    argininosuccinate lyase activity GO:0004056 ISO
    argininosuccinate lyase activity GO:0004056 ISS
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
Biological Process
    urea cycle GO:0000050 IEA
    urea cycle GO:0000050 ISO
    argininosuccinate metabolic process GO:0000053 ISO
    liver development GO:0001889 ISO
    amino acid metabolic process GO:0006520 IMP
    arginine metabolic process GO:0006525 ISO
    L-arginine biosynthetic process GO:0006526 IEA
    L-arginine biosynthetic process GO:0006526 IMP
    L-arginine biosynthetic process GO:0006526 ISO
    L-arginine biosynthetic process GO:0006526 ISS
    locomotory behavior GO:0007626 IMP
    post-embryonic development GO:0009791 IMP
    ammonia assimilation cycle GO:0019676 IMP
    arginine biosynthetic process via ornithine GO:0042450 IBA
    arginine biosynthetic process via ornithine GO:0042450 IEA
    positive regulation of nitric oxide biosynthetic process GO:0045429 IMP
    positive regulation of nitric oxide biosynthetic process GO:0045429 ISO
Subcellular Localization
    mitochondrial outer membrane GO:0005741 ISO
    cytosol GO:0005829 IBA
    neuronal cell body GO:0043025 ISO
    perikaryon GO:0043204 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    cell body fiber GO:0070852 ISO
 Experiment description of studies that identified Asl in sEVs
1
Experiment ID 907
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Asl
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atxn1  
Affinity Capture-MS Mus musculus
2 HSD17B10 3028
Affinity Capture-MS Homo sapiens
3 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml
 Pathways in which Asl is involved
PathwayEvidenceSource
Metabolism IEA Reactome
Metabolism of amino acids and derivatives IEA Reactome
Urea cycle IEA Reactome





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