Gene description for Ckb
Gene name creatine kinase, brain
Gene symbol Ckb
Other names/aliases B-CK
Bck
Ck-3
Ck3
Ckbb
Species Mus musculus
 Database cross references - Ckb
ExoCarta ExoCarta_12709
Entrez Gene 12709
UniProt Q04447  
 Ckb identified in exosomes derived from the following tissue/cell type
Neural stem cells 25242146    
Oligodendrocytes 21136642    
 Gene ontology annotations for Ckb
Molecular Function
    transferase activity GO:0016740 IEA
    protein binding GO:0005515 IPI
    nucleotide binding GO:0000166 IEA
    kinase activity GO:0016301 IEA
    transferase activity, transferring phosphorus-containing groups GO:0016772 IEA
    catalytic activity GO:0003824 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    ATP binding GO:0005524 IEA
    creatine kinase activity GO:0004111 IEA
Biological Process
    phosphorylation GO:0016310 IEA
    cellular chloride ion homeostasis GO:0030644 ISO
Subcellular Localization
    cytoplasm GO:0005737 IEA
    extracellular exosome GO:0070062 ISO
    mitochondrion GO:0005739 IDA
    myelin sheath GO:0043209 IDA
    cell GO:0005623 ISO
 Experiment description of studies that identified Ckb in exosomes
1
Experiment ID 264
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 33
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Ckb
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ckb is involved
PathwayEvidenceSource
Creatine metabolism IEA Reactome





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