Gene description for Ckb
Gene name creatine kinase, brain
Gene symbol Ckb
Other names/aliases B-CK
Bck
Ck-3
Ck3
Ckbb
Species Mus musculus
 Database cross references - Ckb
ExoCarta ExoCarta_12709
Vesiclepedia VP_12709
Entrez Gene 12709
UniProt Q04447  
 Ckb identified in sEVs derived from the following tissue/cell type
Neural stem cells 25242146    
Oligodendrocytes 21136642    
 Gene ontology annotations for Ckb
Molecular Function
    creatine kinase activity GO:0004111 IBA
    creatine kinase activity GO:0004111 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
Biological Process
    intracellular chloride ion homeostasis GO:0030644 ISO
    phosphocreatine biosynthetic process GO:0046314 IBA
    phosphocreatine biosynthetic process GO:0046314 IEA
    futile creatine cycle GO:0140651 IDA
Subcellular Localization
    extracellular space GO:0005615 IBA
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IDA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    dendrite GO:0030425 ISO
    neuronal cell body GO:0043025 ISO
    myelin sheath GO:0043209 HDA
 Experiment description of studies that identified Ckb in sEVs
1
Experiment ID 264
MISEV standards
Biophysical techniques
CD63|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 33
MISEV standards
EM|IEM
Biophysical techniques
Alix|TSG101
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Ckb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Invs  
Affinity Capture-MS Mus musculus
2 Kctd13  
Affinity Capture-MS Mus musculus
3 Cse1l 110750
Co-fractionation Mus musculus
4 Kcna3  
Affinity Capture-MS Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 MAPT  
Reconstituted Complex Homo sapiens
7 Tfe3  
Affinity Capture-MS Mus musculus
8 Mapt  
Affinity Capture-MS Mus musculus
9 HSD17B10 3028
Affinity Capture-MS Homo sapiens
10 Tbcb 66411
Co-fractionation Mus musculus
11 Inadl  
Affinity Capture-MS Mus musculus
12 Mt3  
Affinity Capture-MS Mus musculus
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