Gene description for Alad
Gene name aminolevulinate, delta-, dehydratase
Gene symbol Alad
Other names/aliases ALADH
Lv
Species Mus musculus
 Database cross references - Alad
ExoCarta ExoCarta_17025
Vesiclepedia VP_17025
Entrez Gene 17025
UniProt P10518  
 Alad identified in sEVs derived from the following tissue/cell type
Pancreatic cells 19351151    
 Gene ontology annotations for Alad
Molecular Function
    porphobilinogen synthase activity GO:0004655 IBA
    porphobilinogen synthase activity GO:0004655 IDA
    porphobilinogen synthase activity GO:0004655 IMP
    porphobilinogen synthase activity GO:0004655 ISO
    porphobilinogen synthase activity GO:0004655 ISS
    zinc ion binding GO:0008270 IBA
    zinc ion binding GO:0008270 ISO
    zinc ion binding GO:0008270 ISS
    identical protein binding GO:0042802 ISO
    proteasome core complex binding GO:1904854 IEA
    proteasome core complex binding GO:1904854 ISO
Biological Process
    response to hypoxia GO:0001666 IEA
    protoporphyrinogen IX biosynthetic process GO:0006782 IEA
    heme biosynthetic process GO:0006783 IBA
    heme biosynthetic process GO:0006783 IDA
    heme biosynthetic process GO:0006783 ISO
    heme biosynthetic process GO:0006783 ISS
    heme A biosynthetic process GO:0006784 IDA
    heme A biosynthetic process GO:0006784 IMP
    heme B biosynthetic process GO:0006785 IDA
    heme B biosynthetic process GO:0006785 IMP
    response to oxidative stress GO:0006979 IEA
    response to nutrient GO:0007584 ISO
    response to xenobiotic stimulus GO:0009410 IEA
    response to xenobiotic stimulus GO:0009410 ISO
    response to herbicide GO:0009635 IEA
    response to metal ion GO:0010038 ISO
    response to iron ion GO:0010039 IEA
    response to zinc ion GO:0010043 IEA
    response to zinc ion GO:0010043 ISO
    response to aluminum ion GO:0010044 IEA
    response to ionizing radiation GO:0010212 IEA
    response to vitamin B1 GO:0010266 IEA
    response to selenium ion GO:0010269 IEA
    response to activity GO:0014823 IEA
    response to cobalt ion GO:0032025 IEA
    response to lipopolysaccharide GO:0032496 IEA
    response to vitamin E GO:0033197 IEA
    response to amino acid GO:0043200 IEA
    response to ethanol GO:0045471 IEA
    response to ethanol GO:0045471 ISO
    response to arsenic-containing substance GO:0046685 IEA
    response to arsenic-containing substance GO:0046685 ISO
    response to cadmium ion GO:0046686 IEA
    response to cadmium ion GO:0046686 ISO
    response to mercury ion GO:0046689 IEA
    heme O biosynthetic process GO:0048034 IDA
    heme O biosynthetic process GO:0048034 IMP
    protein homooligomerization GO:0051260 IEA
    protein homooligomerization GO:0051260 ISO
    response to glucocorticoid GO:0051384 IEA
    response to methylmercury GO:0051597 IEA
    response to platinum ion GO:0070541 IEA
    response to fatty acid GO:0070542 IEA
    cellular response to lead ion GO:0071284 IEA
    cellular response to interleukin-4 GO:0071353 IDA
    negative regulation of proteasomal protein catabolic process GO:1901799 IEA
    negative regulation of proteasomal protein catabolic process GO:1901799 ISO
Subcellular Localization
    extracellular space GO:0005615 IEA
    extracellular space GO:0005615 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified Alad in sEVs
1
Experiment ID 188
MISEV standards
EM
Biophysical techniques
GAPDH|UCHL1|HSP90
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Alad
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rprd1b  
Co-fractionation Mus musculus
2 Zpr1  
Co-fractionation Mus musculus
3 HSD17B10 3028
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
4 Wdr4  
Co-fractionation Mus musculus
5 Kcnma1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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