Gene description for Ldhb
Gene name lactate dehydrogenase B
Gene symbol Ldhb
Other names/aliases AI790582
H-Ldh
LDH-B
LDH-H
Ldh-2
Ldh2
Species Mus musculus
 Database cross references - Ldhb
ExoCarta ExoCarta_16832
Vesiclepedia VP_16832
Entrez Gene 16832
UniProt P16125  
 Ldhb identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ldhb
Molecular Function
    lactate dehydrogenase activity GO:0004457 ISO
    L-lactate dehydrogenase activity GO:0004459 IBA
    L-lactate dehydrogenase activity GO:0004459 IDA
    L-lactate dehydrogenase activity GO:0004459 ISO
    kinase binding GO:0019900 ISO
    identical protein binding GO:0042802 ISO
    NAD binding GO:0051287 ISO
Biological Process
    lactate metabolic process GO:0006089 IBA
    lactate metabolic process GO:0006089 ISO
    pyruvate metabolic process GO:0006090 IBA
    pyruvate metabolic process GO:0006090 ISO
    lactate biosynthetic process from pyruvate GO:0019244 IC
    NAD metabolic process GO:0019674 ISO
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IBA
    mitochondrial inner membrane GO:0005743 ISO
    mitochondrial inner membrane GO:0005743 ISS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    myelin sheath GO:0043209 HDA
    membrane raft GO:0045121 ISO
    oxidoreductase complex GO:1990204 ISO
 Experiment description of studies that identified Ldhb in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ldhb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cep290  
Affinity Capture-MS Mus musculus
2 Kctd13  
Affinity Capture-MS Mus musculus
3 MAPT  
Reconstituted Complex Homo sapiens
4 Ldhb 16832
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
5 Tfe3  
Affinity Capture-MS Mus musculus
6 Htt  
Affinity Capture-MS Mus musculus
7 HSD17B10 3028
Affinity Capture-MS Homo sapiens
8 Ubfd1  
Co-fractionation Mus musculus
9 Cisd1  
Co-fractionation Mus musculus
10 Ndufs2  
Co-fractionation Mus musculus
11 Suclg1  
Co-fractionation Mus musculus
12 Kcnma1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Ldhb is involved
PathwayEvidenceSource
Aerobic respiration and respiratory electron transport IEA Reactome
Metabolism IEA Reactome
Pyruvate metabolism IEA Reactome





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