Gene description for Gstp1
Gene name glutathione S-transferase, pi 1
Gene symbol Gstp1
Other names/aliases GstpiB
Species Mus musculus
 Database cross references - Gstp1
ExoCarta ExoCarta_14870
Vesiclepedia VP_14870
Entrez Gene 14870
UniProt P19157  
 Gstp1 identified in exosomes derived from the following tissue/cell type
Brain cancer cells 19109410    
Colon cancer cells 37309723    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Gstp1
Molecular Function
    drug binding GO:0008144 ISO
    S-nitrosoglutathione binding GO:0035730 ISO
    dinitrosyl-iron complex binding GO:0035731 ISO
    kinase regulator activity GO:0019207 IMP
    glutathione binding GO:0043295 ISO
    glutathione transferase activity GO:0004364 ISO
    JUN kinase binding GO:0008432 IPI
    protein binding GO:0005515 IPI
    transferase activity GO:0016740 IEA
Biological Process
    response to reactive oxygen species GO:0000302 IDA
    regulation of stress-activated MAPK cascade GO:0032872 IDA
    negative regulation of acute inflammatory response GO:0002674 NAS
    negative regulation of stress-activated MAPK cascade GO:0032873 IDA
    metabolic process GO:0008152 IEA
    negative regulation of tumor necrosis factor production GO:0032720 IDA
    negative regulation of neutrophil aggregation GO:2000429 NAS
    positive regulation of superoxide anion generation GO:0032930 IMP
    common myeloid progenitor cell proliferation GO:0035726 IMP
    negative regulation of biosynthetic process GO:0009890 IDA
    regulation of ERK1 and ERK2 cascade GO:0070372 IDA
    negative regulation of JUN kinase activity GO:0043508 IMP
    negative regulation of fibroblast proliferation GO:0048147 IMP
    negative regulation of interleukin-1 beta production GO:0032691 IDA
    negative regulation of nitric-oxide synthase biosynthetic process GO:0051771 IDA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IDA
    negative regulation of peroxidase activity GO:2000469 IDA
    negative regulation of apoptotic process GO:0043066 TAS
    negative regulation of I-kappaB kinase/NF-kappaB signaling GO:0043124 IDA
    cellular response to lipopolysaccharide GO:0071222 IDA
    negative regulation of monocyte chemotactic protein-1 production GO:0071638 IDA
    negative regulation of leukocyte proliferation GO:0070664 IMP
    glutathione metabolic process GO:0006749 IDA
Subcellular Localization
    intracellular GO:0005622 ISO
    TRAF2-GSTP1 complex GO:0097057 ISO
    plasma membrane GO:0005886 ISO
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    nucleus GO:0005634 ISO
    vesicle GO:0031982 ISO
    extracellular exosome GO:0070062 ISO
    extracellular space GO:0005615 ISO
    mitochondrion GO:0005739 ISO
    protein complex GO:0043234 IDA
 Experiment description of studies that identified Gstp1 in exosomes
1
Experiment ID 26
MISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|GAPDH|HSP70|CD9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
2
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 33
MISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
Alix|TSG101
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 188
MISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Gstp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Gstp1 is involved
PathwayEvidenceSource
Detoxification of Reactive Oxygen Species IEA Reactome
Glutathione conjugation IEA Reactome





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