Gene description for Gstp1
Gene name glutathione S-transferase, pi 1
Gene symbol Gstp1
Other names/aliases GstpiB
Species Mus musculus
 Database cross references - Gstp1
ExoCarta ExoCarta_14870
Vesiclepedia VP_14870
Entrez Gene 14870
UniProt P19157  
 Gstp1 identified in sEVs derived from the following tissue/cell type
Brain cancer cells 19109410    
Colon cancer cells 37309723    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Gstp1
Molecular Function
    glutathione transferase activity GO:0004364 IBA
    glutathione transferase activity GO:0004364 IDA
    glutathione transferase activity GO:0004364 ISO
    glutathione peroxidase activity GO:0004602 ISO
    fatty acid binding GO:0005504 ISO
    JUN kinase binding GO:0008432 IPI
    toxic substance binding GO:0015643 ISO
    kinase regulator activity GO:0019207 IMP
    protein kinase binding GO:0019901 ISO
    S-nitrosoglutathione binding GO:0035730 ISO
    dinitrosyl-iron complex binding GO:0035731 ISO
    organic cyclic compound binding GO:0097159 ISO
Biological Process
    response to reactive oxygen species GO:0000302 IDA
    negative regulation of acute inflammatory response GO:0002674 NAS
    negative regulation of protein kinase activity GO:0006469 ISO
    prostaglandin metabolic process GO:0006693 ISO
    prostaglandin metabolic process GO:0006693 ISS
    glutathione metabolic process GO:0006749 IBA
    glutathione metabolic process GO:0006749 IDA
    glutathione metabolic process GO:0006749 ISO
    xenobiotic metabolic process GO:0006805 ISO
    xenobiotic metabolic process GO:0006805 ISS
    response to toxic substance GO:0009636 ISS
    negative regulation of biosynthetic process GO:0009890 ISO
    oligodendrocyte development GO:0014003 ISS
    animal organ regeneration GO:0031100 ISS
    response to nutrient levels GO:0031667 ISS
    response to estradiol GO:0032355 ISS
    negative regulation of interleukin-1 beta production GO:0032691 IDA
    negative regulation of interleukin-1 beta production GO:0032691 ISO
    negative regulation of tumor necrosis factor production GO:0032720 IDA
    negative regulation of tumor necrosis factor production GO:0032720 ISO
    cellular response to insulin stimulus GO:0032869 ISS
    regulation of stress-activated MAPK cascade GO:0032872 IDA
    negative regulation of stress-activated MAPK cascade GO:0032873 IDA
    positive regulation of superoxide anion generation GO:0032930 IMP
    response to L-ascorbic acid GO:0033591 ISS
    common myeloid progenitor cell proliferation GO:0035726 IMP
    negative regulation of apoptotic process GO:0043066 TAS
    negative regulation of canonical NF-kappaB signal transduction GO:0043124 IDA
    response to amino acid GO:0043200 ISS
    negative regulation of MAP kinase activity GO:0043407 ISO
    negative regulation of JUN kinase activity GO:0043508 ISO
    linoleic acid metabolic process GO:0043651 ISO
    response to ethanol GO:0045471 ISS
    negative regulation of fibroblast proliferation GO:0048147 IMP
    hepoxilin biosynthetic process GO:0051122 ISO
    hepoxilin biosynthetic process GO:0051122 ISS
    regulation of ERK1 and ERK2 cascade GO:0070372 IDA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IDA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 ISO
    negative regulation of leukocyte proliferation GO:0070664 IMP
    cellular response to lipopolysaccharide GO:0071222 IDA
    cellular response to epidermal growth factor stimulus GO:0071364 ISS
    cellular response to glucocorticoid stimulus GO:0071385 ISS
    cellular response to cell-matrix adhesion GO:0071460 ISS
    negative regulation of monocyte chemotactic protein-1 production GO:0071638 IDA
    negative regulation of monocyte chemotactic protein-1 production GO:0071638 ISO
    negative regulation of smooth muscle cell chemotaxis GO:0071672 ISO
    glutathione derivative biosynthetic process GO:1901687 ISO
    glutathione derivative biosynthetic process GO:1901687 ISS
    negative regulation of vascular associated smooth muscle cell proliferation GO:1904706 ISO
    negative regulation of neutrophil aggregation GO:2000429 NAS
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 ISO
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 ISS
Subcellular Localization
    nucleus GO:0005634 IEA
    mitochondrion GO:0005739 IEA
    mitochondrion GO:0005739 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    protein-containing complex GO:0032991 IDA
    TRAF2-GSTP1 complex GO:0097057 ISO
 Experiment description of studies that identified Gstp1 in sEVs
1
Experiment ID 26
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|GAPDH|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors "Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD"
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
2
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Gstp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancc  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
2 Atxn1  
Affinity Capture-MS Mus musculus
3 Eed  
Affinity Capture-MS Mus musculus
4 Nlrp3  
Affinity Capture-MS Mus musculus
5 MAPT  
Reconstituted Complex Homo sapiens
6 Tfe3  
Affinity Capture-MS Mus musculus
7 Tia1  
Affinity Capture-MS Mus musculus
8 HSD17B10 3028
Affinity Capture-MS Homo sapiens
9 Kctd13  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here