Gene description for Ass1
Gene name argininosuccinate synthetase 1
Gene symbol Ass1
Other names/aliases AA408052
ASS
Ass-1
fold
Species Mus musculus
 Database cross references - Ass1
ExoCarta ExoCarta_11898
Vesiclepedia VP_11898
Entrez Gene 11898
UniProt P16460  
 Ass1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Plasma 32132711    
Plasma 32132711    
Plasma 32132711    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ass1
Molecular Function
    argininosuccinate synthase activity GO:0004055 IBA
    argininosuccinate synthase activity GO:0004055 ISO
    argininosuccinate synthase activity GO:0004055 ISS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    toxic substance binding GO:0015643 IEA
    toxic substance binding GO:0015643 ISO
    amino acid binding GO:0016597 IEA
    amino acid binding GO:0016597 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
Biological Process
    urea cycle GO:0000050 IBA
    urea cycle GO:0000050 IEA
    urea cycle GO:0000050 ISO
    urea cycle GO:0000050 ISS
    citrulline metabolic process GO:0000052 IEA
    citrulline metabolic process GO:0000052 ISO
    argininosuccinate metabolic process GO:0000053 IBA
    argininosuccinate metabolic process GO:0000053 IEA
    argininosuccinate metabolic process GO:0000053 ISO
    kidney development GO:0001822 IEA
    liver development GO:0001889 IEA
    L-arginine biosynthetic process GO:0006526 IBA
    L-arginine biosynthetic process GO:0006526 IEA
    L-arginine biosynthetic process GO:0006526 ISO
    L-arginine biosynthetic process GO:0006526 ISS
    aspartate metabolic process GO:0006531 IEA
    aspartate metabolic process GO:0006531 ISO
    acute-phase response GO:0006953 IEA
    midgut development GO:0007494 IEA
    response to nutrient GO:0007584 IEA
    circadian rhythm GO:0007623 IDA
    circadian rhythm GO:0007623 ISO
    response to xenobiotic stimulus GO:0009410 IEA
    response to zinc ion GO:0010043 IEA
    response to zinc ion GO:0010043 ISO
    response to mycotoxin GO:0010046 IEA
    response to mycotoxin GO:0010046 ISO
    response to estradiol GO:0032355 IEA
    positive regulation of nitric oxide biosynthetic process GO:0045429 IEA
    positive regulation of nitric oxide biosynthetic process GO:0045429 ISO
    response to glucocorticoid GO:0051384 ISO
    response to growth hormone GO:0060416 IEA
    diaphragm development GO:0060539 IEA
    cellular response to lipopolysaccharide GO:0071222 IEA
    cellular response to amino acid stimulus GO:0071230 IEA
    cellular response to ammonium ion GO:0071242 IEA
    cellular response to cAMP GO:0071320 IEA
    cellular response to type II interferon GO:0071346 IEA
    cellular response to tumor necrosis factor GO:0071356 IEA
    cellular response to glucagon stimulus GO:0071377 IEA
    cellular response to oleic acid GO:0071400 IEA
    cellular response to amine stimulus GO:0071418 IEA
    cellular response to laminar fluid shear stress GO:0071499 IEA
    cellular response to laminar fluid shear stress GO:0071499 ISO
    cellular response to dexamethasone stimulus GO:0071549 IEA
    negative regulation of leukocyte cell-cell adhesion GO:1903038 IEA
    negative regulation of leukocyte cell-cell adhesion GO:1903038 ISO
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial outer membrane GO:0005741 IEA
    mitochondrial outer membrane GO:0005741 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    neuronal cell body GO:0043025 ISO
    perikaryon GO:0043204 IEA
    perikaryon GO:0043204 ISO
    myelin sheath GO:0043209 HDA
    cell body fiber GO:0070852 IEA
    cell body fiber GO:0070852 ISO
 Experiment description of studies that identified Ass1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 333
MISEV standards
✘ Biophysical techniques
✔
Cd81|Cd9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32132711    
Organism Mus musculus
Experiment description Decoy exosomes provide protection against bacterial toxins
Authors "Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K."
Journal name Nature
Publication year 2020
Sample Plasma
Sample name Plasma - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
3
Experiment ID 334
MISEV standards
✘ Biophysical techniques
✔
Cd81|Cd9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32132711    
Organism Mus musculus
Experiment description Decoy exosomes provide protection against bacterial toxins
Authors "Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K."
Journal name Nature
Publication year 2020
Sample Plasma
Sample name Plasma - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 335
MISEV standards
✘ Biophysical techniques
✔
Cd81|Cd9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32132711    
Organism Mus musculus
Experiment description Decoy exosomes provide protection against bacterial toxins
Authors "Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K."
Journal name Nature
Publication year 2020
Sample Plasma
Sample name Plasma - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ass1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tfe3  
Affinity Capture-MS Mus musculus
2 Tnfaip8  
Co-fractionation Mus musculus
3 Fancd2  
Affinity Capture-MS Mus musculus
4 HSD17B10 3028
Affinity Capture-MS Homo sapiens
5 Sstr3  
Proximity Label-MS Mus musculus
View the network image/svg+xml
 Pathways in which Ass1 is involved
PathwayEvidenceSource
Metabolism IEA Reactome
Metabolism of amino acids and derivatives IEA Reactome
Urea cycle IEA Reactome





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