Gene description for Paics
Gene name phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoribosylaminoimidazole, succinocarboxamide synthetase
Gene symbol Paics
Other names/aliases 2610511I09Rik
ADE2H1
AIRC
PAIS
Species Mus musculus
 Database cross references - Paics
ExoCarta ExoCarta_67054
Vesiclepedia VP_67054
Entrez Gene 67054
UniProt Q9DCL9  
 Paics identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Paics
Molecular Function
    phosphoribosylaminoimidazole carboxylase activity GO:0004638 ISO
    phosphoribosylaminoimidazole carboxylase activity GO:0004638 ISS
    phosphoribosylaminoimidazolesuccinocarboxamide synthase activity GO:0004639 IBA
    phosphoribosylaminoimidazolesuccinocarboxamide synthase activity GO:0004639 ISO
    phosphoribosylaminoimidazolesuccinocarboxamide synthase activity GO:0004639 ISS
    ATP binding GO:0005524 IEA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
Biological Process
    GMP biosynthetic process GO:0006177 ISO
    'de novo' IMP biosynthetic process GO:0006189 IBA
    'de novo' IMP biosynthetic process GO:0006189 IEA
    'de novo' IMP biosynthetic process GO:0006189 ISO
    purine nucleobase biosynthetic process GO:0009113 ISO
    purine nucleobase biosynthetic process GO:0009113 ISS
    'de novo' AMP biosynthetic process GO:0044208 ISO
    'de novo' XMP biosynthetic process GO:0097294 ISO
Subcellular Localization
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Paics in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Paics
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mettl21e  
Affinity Capture-MS Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 Atxn1  
Affinity Capture-MS Mus musculus
4 GLP1R  
PCA Homo sapiens
5 Foxp3  
Affinity Capture-MS Mus musculus
6 Eed  
Affinity Capture-MS Mus musculus
7 Traf3  
Proximity Label-MS Mus musculus
8 Fitm2  
Positive Genetic Mus musculus
9 Atg16l1  
Affinity Capture-MS Mus musculus
10 HSD17B10 3028
Affinity Capture-MS Homo sapiens
11 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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