Gene description for Mdh1
Gene name malate dehydrogenase 1, NAD (soluble)
Gene symbol Mdh1
Other names/aliases B230377B03Rik
D17921
MDH-s
MDHA
Mor-2
Mor2
Species Mus musculus
 Database cross references - Mdh1
ExoCarta ExoCarta_17449
Entrez Gene 17449
UniProt P14152  
 Mdh1 identified in exosomes derived from the following tissue/cell type
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
 Gene ontology annotations for Mdh1
Molecular Function
    malate dehydrogenase activity GO:0016615 ISO
    oxidoreductase activity GO:0016491 IEA
    catalytic activity GO:0003824 IEA
    NAD binding GO:0051287 ISO
    oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor GO:0016616 IEA
    L-malate dehydrogenase activity GO:0030060 ISO
Biological Process
    oxaloacetate metabolic process GO:0006107 ISO
    NADH metabolic process GO:0006734 ISO
    malate metabolic process GO:0006108 ISO
    carboxylic acid metabolic process GO:0019752 IEA
    activation of mitophagy in response to mitochondrial depolarization GO:0098779 ISO
    tricarboxylic acid cycle GO:0006099 IEA
    NAD metabolic process GO:0019674 ISO
    positive regulation of defense response to virus by host GO:0002230 ISO
    carbohydrate metabolic process GO:0005975 IEA
    oxidation-reduction process GO:0055114 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    extracellular exosome GO:0070062 ISO
    cytosol GO:0005829 ISO
    extracellular space GO:0005615 ISO
    mitochondrion GO:0005739 IDA
    centrosome GO:0005813 ISO
    myelin sheath GO:0043209 IDA
 Experiment description of studies that identified Mdh1 in exosomes
1
Experiment ID 33
ISEV standards
EM|IEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
2
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Mdh1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Mdh1 is involved
PathwayEvidenceSource
Gluconeogenesis IEA Reactome





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