Gene description for Mdh1
Gene name malate dehydrogenase 1, NAD (soluble)
Gene symbol Mdh1
Other names/aliases B230377B03Rik
D17921
MDH-s
MDHA
Mor-2
Mor2
Species Mus musculus
 Database cross references - Mdh1
ExoCarta ExoCarta_17449
Vesiclepedia VP_17449
Entrez Gene 17449
UniProt P14152  
 Mdh1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Mdh1
Molecular Function
    malate dehydrogenase activity GO:0016615 ISO
    L-malate dehydrogenase (NAD+) activity GO:0030060 IBA
    L-malate dehydrogenase (NAD+) activity GO:0030060 IDA
    L-malate dehydrogenase (NAD+) activity GO:0030060 IMP
    L-malate dehydrogenase (NAD+) activity GO:0030060 ISO
    hydroxyphenylpyruvate reductase activity GO:0047995 IEA
    NAD binding GO:0051287 ISO
Biological Process
    gluconeogenesis GO:0006094 TAS
    tricarboxylic acid cycle GO:0006099 IBA
    tricarboxylic acid cycle GO:0006099 IEA
    oxaloacetate metabolic process GO:0006107 IBA
    oxaloacetate metabolic process GO:0006107 ISO
    malate metabolic process GO:0006108 IBA
    malate metabolic process GO:0006108 IEA
    malate metabolic process GO:0006108 ISO
    NADH metabolic process GO:0006734 IBA
    NADH metabolic process GO:0006734 ISO
    NADP metabolic process GO:0006739 ISO
    NAD metabolic process GO:0019674 ISO
    malate-aspartate shuttle GO:0043490 IMP
    glyceraldehyde-3-phosphate biosynthetic process GO:0046166 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 HDA
    centrosome GO:0005813 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    myelin sheath GO:0043209 HDA
 Experiment description of studies that identified Mdh1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Mdh1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tmem173  
Proximity Label-MS Mus musculus
2 Sstr3  
Proximity Label-MS Mus musculus
3 Kcna3  
Affinity Capture-MS Mus musculus
4 Kctd13  
Affinity Capture-MS Mus musculus
5 MAPT  
Reconstituted Complex Homo sapiens
6 Mdh1 17449
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
7 Tia1  
Affinity Capture-MS Mus musculus
8 HSD17B10 3028
Affinity Capture-MS Homo sapiens
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