Gene description for Gpi1
Gene name glucose phosphate isomerase 1
Gene symbol Gpi1
Other names/aliases Amf
Gpi
Gpi-1
Gpi-1r
Gpi-1s
Gpi-1t
Gpi1-r
Gpi1-s
Gpi1-t
Gpi1s
MF
NK
NK/GPI
Nlk
Org
Pgi
Phi
Species Mus musculus
 Database cross references - Gpi1
ExoCarta ExoCarta_14751
Entrez Gene 14751
UniProt P06745  
 Gpi1 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
Pancreatic cells 19351151    
 Gene ontology annotations for Gpi1
Molecular Function
    protein binding GO:0005515 IPI
    isomerase activity GO:0016853 IEA
    glucose-6-phosphate isomerase activity GO:0004347 ISO
    intramolecular transferase activity GO:0016866 ISO
    monosaccharide binding GO:0048029 ISO
    cytokine activity GO:0005125 IEA
    growth factor activity GO:0008083 IEA
Biological Process
    gluconeogenesis GO:0006094 IEA
    glucose homeostasis GO:0042593 IMP
    mesoderm formation GO:0001707 IMP
    methylglyoxal biosynthetic process GO:0019242 ISO
    in utero embryonic development GO:0001701 IMP
    angiogenesis GO:0001525 IEA
    erythrocyte homeostasis GO:0034101 IMP
    carbohydrate metabolic process GO:0005975 ISO
    glucose 6-phosphate metabolic process GO:0051156 ISO
    glycolytic process GO:0006096 IMP
    glycolytic process through glucose-6-phosphate GO:0061620 IC
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 ISO
    aldehyde catabolic process GO:0046185 ISO
    negative regulation of neuron apoptotic process GO:0043524 ISO
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    ciliary membrane GO:0060170 IDA
    extracellular region GO:0005576 IEA
    membrane GO:0016020 ISO
    plasma membrane GO:0005886 ISO
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    extracellular space GO:0005615 IEA
    nucleoplasm GO:0005654 ISO
    myelin sheath GO:0043209 IDA
    neuron projection GO:0043005 ISO
 Experiment description of studies that identified Gpi1 in exosomes
1
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
5
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
6
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Gpi1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Gpi1 is involved
PathwayEvidenceSource
Gluconeogenesis IEA Reactome
Glycolysis IEA Reactome
TP53 Regulates Metabolic Genes IEA Reactome





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