Gene description for CHST1
Gene name carbohydrate (keratan sulfate Gal-6) sulfotransferase 1
Gene symbol CHST1
Other names/aliases C6ST
GST-1
KS6ST
KSGal6ST
KSST
Species Homo sapiens
 Database cross references - CHST1
ExoCarta ExoCarta_8534
Entrez Gene 8534
HGNC 1969
MIM 603797
UniProt O43916  
 CHST1 identified in exosomes derived from the following tissue/cell type
Tracheobronchial cells 19190083    
 Gene ontology annotations for CHST1
Molecular Function
    keratan sulfotransferase activity GO:0045130 IDA
    sulfotransferase activity GO:0008146 IDA
Biological Process
    keratan sulfate metabolic process GO:0042339 TAS
    inflammatory response GO:0006954 IEA
    sulfur compound metabolic process GO:0006790 IDA
    polysaccharide metabolic process GO:0005976 TAS
    small molecule metabolic process GO:0044281 TAS
    keratan sulfate biosynthetic process GO:0018146 TAS
    glycosaminoglycan metabolic process GO:0030203 TAS
    carbohydrate metabolic process GO:0005975 TAS
    galactose metabolic process GO:0006012 IDA
Subcellular Localization
    integral component of membrane GO:0016021 NAS
    Golgi membrane GO:0000139 TAS
 Experiment description of studies that identified CHST1 in exosomes
1
Experiment ID 34
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD63|MHCI|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 19190083    
Organism Homo sapiens
Experiment description Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense.
Authors Kesimer M, Scull M, Brighton B, Demaria G, Burns K, O'Neal W, Pickles RJ, Sheehan JK
Journal name FASEB
Publication year 2009
Sample Tracheobronchial cells
Sample name Tracheobronchial epithelial cells
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.16 -1.18 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
FACS
 Protein-protein interactions for CHST1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SFN 2810
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which CHST1 is involved
PathwayEvidenceSource
Keratan sulfate biosynthesis TAS Reactome





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