Gene description for RYR1
Gene name ryanodine receptor 1 (skeletal)
Gene symbol RYR1
Other names/aliases CCO
MHS
MHS1
PPP1R137
RYDR
RYR
RYR-1
SKRR
Species Homo sapiens
 Database cross references - RYR1
ExoCarta ExoCarta_6261
Vesiclepedia VP_6261
Entrez Gene 6261
HGNC 10483
MIM 180901
UniProt P21817  
 RYR1 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 25844599    
Urine 19056867    
 Gene ontology annotations for RYR1
Molecular Function
    calmodulin binding GO:0005516 ISS
    protease binding GO:0002020 IEA
    voltage-gated calcium channel activity GO:0005245 ISS
    calcium channel activity GO:0005262 ISS
    calcium-release channel activity GO:0015278 TAS
    protein binding GO:0005515 IPI
    ryanodine-sensitive calcium-release channel activity GO:0005219 TAS
Biological Process
    outflow tract morphogenesis GO:0003151 ISS
    cellular response to caffeine GO:0071313 ISS
    skeletal muscle fiber development GO:0048741 ISS
    muscle contraction GO:0006936 ISS
    cytosolic calcium ion homeostasis GO:0051480 ISS
    response to hypoxia GO:0001666 IDA
    transmembrane transport GO:0055085 TAS
    ossification involved in bone maturation GO:0043931 ISS
    calcium ion transport GO:0006816 ISS
    ion transmembrane transport GO:0034220 TAS
    release of sequestered calcium ion into cytosol by sarcoplasmic reticulum GO:0014808 ISS
    calcium ion transmembrane transport GO:0070588 IEA
    response to caffeine GO:0031000 ISS
    skin development GO:0043588 ISS
    release of sequestered calcium ion into cytosol GO:0051209 ISS
Subcellular Localization
    I band GO:0031674 IDA
    junctional membrane complex GO:0030314 IEA
    smooth endoplasmic reticulum GO:0005790 IEA
    plasma membrane GO:0005886 IDA
    integral component of plasma membrane GO:0005887 TAS
    junctional sarcoplasmic reticulum membrane GO:0014701 TAS
    extracellular exosome GO:0070062 IDA
    cell cortex GO:0005938 IDA
    cytoplasm GO:0005737 IDA
    terminal cisterna GO:0014802 ISS
    sarcoplasmic reticulum GO:0016529 ISS
    T-tubule GO:0030315 IEA
    sarcoplasmic reticulum membrane GO:0033017 TAS
    cytosol GO:0005829 ISS
 Experiment description of studies that identified RYR1 in exosomes
1
Experiment ID 211
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
MISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 274
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
EV Enriched markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
4
Experiment ID 63
MISEV standards
✘ EV Biophysical techniques
✔
AQP2
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for RYR1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TRDN 10345
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
2 RYR2 6262
Affinity Capture-Western Homo sapiens
3 HOMER3 9454
Invivo Homo sapiens
Invitro Homo sapiens
4 CACNA1S 779
Invivo Homo sapiens
5 RYR1 6261
Invivo Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
6 S100A1 6271
Invitro Homo sapiens
7 HOMER2  
Invivo Homo sapiens
Invitro Homo sapiens
8 CALM1 801
Invitro Homo sapiens
9 FKBP1A 2280
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
10 HOMER1  
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which RYR1 is involved
PathwayEvidenceSource
Stimuli-sensing channels TAS Reactome





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