Gene description for Vim
Gene name vimentin
Gene symbol Vim
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Vim
ExoCarta ExoCarta_81818
Vesiclepedia VP_81818
Entrez Gene 81818
UniProt P31000  
 Vim identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Vim
Molecular Function
    RNA binding GO:0003723 ISO
    double-stranded RNA binding GO:0003725 IEA
    double-stranded RNA binding GO:0003725 ISO
    structural molecule activity GO:0005198 TAS
    structural constituent of cytoskeleton GO:0005200 IBA
    structural constituent of cytoskeleton GO:0005200 IEA
    structural constituent of cytoskeleton GO:0005200 ISO
    structural constituent of cytoskeleton GO:0005200 ISS
    structural constituent of eye lens GO:0005212 IEA
    structural constituent of eye lens GO:0005212 ISO
    protein binding GO:0005515 IPI
    kinase binding GO:0019900 IPI
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    protein phosphatase 2A binding GO:0051721 IDA
    molecular adaptor activity GO:0060090 ISO
    scaffold protein binding GO:0097110 IEA
    scaffold protein binding GO:0097110 ISO
    keratin filament binding GO:1990254 IEA
    keratin filament binding GO:1990254 ISO
    protein tyrosine kinase binding GO:1990782 IPI
Biological Process
    response to zinc ion GO:0010043 IEP
    response to selenium ion GO:0010269 IEP
    positive regulation of gene expression GO:0010628 IEA
    positive regulation of gene expression GO:0010628 ISO
    positive regulation of epithelial cell migration GO:0010634 ISO
    positive regulation of epithelial cell migration GO:0010634 ISS
    negative regulation of neuron projection development GO:0010977 IEA
    negative regulation of neuron projection development GO:0010977 ISO
    astrocyte development GO:0014002 IEA
    astrocyte development GO:0014002 ISO
    neuron projection development GO:0031175 IEA
    neuron projection development GO:0031175 ISO
    response to estradiol GO:0032355 IEP
    positive regulation of collagen biosynthetic process GO:0032967 IEA
    positive regulation of collagen biosynthetic process GO:0032967 ISO
    response to vitamin D GO:0033280 IEP
    skeletal muscle tissue regeneration GO:0043403 IEP
    regulation of mRNA stability GO:0043488 IEA
    regulation of mRNA stability GO:0043488 ISO
    cellular response to fibroblast growth factor stimulus GO:0044344 IEP
    intermediate filament-based process GO:0045103 ISO
    intermediate filament organization GO:0045109 IBA
    intermediate filament organization GO:0045109 IEA
    intermediate filament organization GO:0045109 ISO
    intermediate filament organization GO:0045109 ISO
    intermediate filament organization GO:0045109 ISS
    fat cell differentiation GO:0045444 IEP
    decidualization GO:0046697 IEP
    regulation of axonogenesis GO:0050770 IDA
    Bergmann glial cell differentiation GO:0060020 IEA
    Bergmann glial cell differentiation GO:0060020 ISO
    positive regulation of glial cell proliferation GO:0060252 IMP
    lens fiber cell development GO:0070307 IEA
    lens fiber cell development GO:0070307 ISO
    cellular response to lipopolysaccharide GO:0071222 IEA
    cellular response to lipopolysaccharide GO:0071222 ISO
    cellular response to lipopolysaccharide GO:0071222 ISS
    cellular response to muramyl dipeptide GO:0071225 IEA
    cellular response to muramyl dipeptide GO:0071225 ISO
    cellular response to muramyl dipeptide GO:0071225 ISS
    cellular response to type II interferon GO:0071346 IEA
    cellular response to type II interferon GO:0071346 ISO
    regulation of Schwann cell migration GO:1900147 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    peroxisome GO:0005777 IEA
    peroxisome GO:0005777 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    cytoskeleton GO:0005856 ISO
    intermediate filament GO:0005882 IBA
    intermediate filament GO:0005882 IEA
    intermediate filament GO:0005882 ISO
    intermediate filament GO:0005882 ISS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    nuclear matrix GO:0016363 IEA
    axon GO:0030424 IBA
    axon GO:0030424 IDA
    cell leading edge GO:0031252 IEA
    cell leading edge GO:0031252 ISO
    cell projection GO:0042995 IDA
    cell projection GO:0042995 ISO
    neuron projection GO:0043005 IEA
    neuron projection GO:0043005 ISO
    cell body GO:0044297 IDA
    type III intermediate filament GO:0045098 TAS
    intermediate filament cytoskeleton GO:0045111 ISO
    phagocytic vesicle GO:0045335 IEA
    phagocytic vesicle GO:0045335 ISO
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified Vim in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Vim
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Gja1  
Affinity Capture-MS Rattus norvegicus
2 PPL 5493
Co-fractionation Homo sapiens
3 Rad23b  
Affinity Capture-MS Rattus norvegicus
4 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
5 DYNLL1 8655
Affinity Capture-MS Homo sapiens
6 Pla2g4a  
Far Western Mus musculus
7 Sumo3  
Affinity Capture-MS Rattus norvegicus
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