Gene description for Myo10
Gene name myosin X
Gene symbol Myo10
Other names/aliases AW048724
D15Ertd600e
mKIAA0799
Species Mus musculus
 Database cross references - Myo10
ExoCarta ExoCarta_17909
Vesiclepedia VP_17909
Entrez Gene 17909
UniProt F8VQB6  
 Myo10 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Myo10
Molecular Function
    microfilament motor activity GO:0000146 ISS
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IEA
    ATP binding GO:0005524 IEA
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 IBA
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 ISS
    spectrin binding GO:0030507 ISO
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 ISO
    actin filament binding GO:0051015 ISS
    plus-end directed microfilament motor activity GO:0060002 IBA
    plus-end directed microfilament motor activity GO:0060002 ISS
Biological Process
    regulation of cell shape GO:0008360 IBA
    regulation of cell shape GO:0008360 ISO
    regulation of cell shape GO:0008360 ISS
    positive regulation of cell-cell adhesion GO:0022409 IGI
    cytoskeleton-dependent intracellular transport GO:0030705 IBA
    cytoskeleton-dependent intracellular transport GO:0030705 ISS
    cell motility GO:0048870 IC
    regulation of filopodium assembly GO:0051489 IBA
    regulation of filopodium assembly GO:0051489 ISO
    regulation of filopodium assembly GO:0051489 ISS
Subcellular Localization
    ruffle GO:0001726 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 ISO
    cell cortex GO:0005938 IEA
    myosin complex GO:0016459 IEA
    lamellipodium GO:0030027 ISO
    filopodium GO:0030175 IBA
    filopodium GO:0030175 IDA
    filopodium membrane GO:0031527 IEA
    filopodium tip GO:0032433 ISS
    neuron projection GO:0043005 IDA
    neuronal cell body GO:0043025 IDA
 Experiment description of studies that identified Myo10 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Myo10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DYNLL1 8655
Affinity Capture-MS Homo sapiens
2 Zbtb7b  
Affinity Capture-MS Mus musculus
3 Pan2  
Co-fractionation Mus musculus
View the network image/svg+xml



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