Gene description for Tuba1a
Gene name tubulin, alpha 1A
Gene symbol Tuba1a
Other names/aliases Tuba1
Species Rattus norvegicus
 Database cross references - Tuba1a
ExoCarta ExoCarta_64158
Vesiclepedia VP_64158
Entrez Gene 64158
UniProt P68370  
 Tuba1a identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Cortical neurones 16446100    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Tuba1a
Molecular Function
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    structural constituent of cytoskeleton GO:0005200 IEA
    protein domain specific binding GO:0019904 IDA
    GTPase activity GO:0003924 IEA
    protein heterodimerization activity GO:0046982 TAS
Biological Process
    metabolic process GO:0008152 IEA
    microtubule-based process GO:0007017 TAS
    protein polymerization GO:0051258 IEA
Subcellular Localization
    recycling endosome GO:0055037 ISO
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    myelin sheath GO:0043209 ISO
    nucleus GO:0005634 ISO
    cytoplasmic microtubule GO:0005881 ISO
    microtubule GO:0005874 TAS
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Tuba1a in exosomes
1
Experiment ID 225
MISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 30
MISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|FLOT
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16446100    
Organism Rattus norvegicus
Experiment description Exosomes are released by cultured cortical neurones.
Authors Faure J, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R
Journal name MCN
Publication year 2006
Sample Cortical neurones
Sample name Cortical neurones
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 94
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Tuba1a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DYNLL1 8655
Affinity Capture-MS Homo sapiens
2 Epn1  
Reconstituted Complex Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
3 Rac1 363875
Co-purification Rattus norvegicus
4 Epn2  
Affinity Capture-Western Rattus norvegicus
5 MLLT10  
Affinity Capture-MS Homo sapiens
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