Gene description for Tuba1a
Gene name tubulin, alpha 1A
Gene symbol Tuba1a
Other names/aliases Tuba1
Species Rattus norvegicus
 Database cross references - Tuba1a
ExoCarta ExoCarta_64158
Vesiclepedia VP_64158
Entrez Gene 64158
UniProt P68370  
 Tuba1a identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Cortical neurones 16446100    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Tuba1a
Molecular Function
    structural constituent of cytoskeleton GO:0005200 IBA
    structural constituent of cytoskeleton GO:0005200 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    hydrolase activity GO:0016787 IEA
    protein domain specific binding GO:0019904 IDA
    identical protein binding GO:0042802 ISO
    protein-containing complex binding GO:0044877 ISO
    protein-containing complex binding GO:0044877 TAS
    metal ion binding GO:0046872 IEA
    protein heterodimerization activity GO:0046982 ISO
Biological Process
    microtubule cytoskeleton organization GO:0000226 IBA
    microtubule cytoskeleton organization GO:0000226 ISO
    mitotic cell cycle GO:0000278 IBA
    neuron migration GO:0001764 ISO
    startle response GO:0001964 ISO
    intracellular protein transport GO:0006886 ISO
    cytoskeleton organization GO:0007010 IEA
    microtubule-based process GO:0007017 IEA
    microtubule-based process GO:0007017 TAS
    centrosome cycle GO:0007098 ISO
    smoothened signaling pathway GO:0007224 ISO
    memory GO:0007613 ISO
    locomotory behavior GO:0007626 ISO
    adult locomotory behavior GO:0008344 ISO
    visual learning GO:0008542 ISO
    response to mechanical stimulus GO:0009612 ISO
    glial cell differentiation GO:0010001 ISO
    gene expression GO:0010467 ISO
    dentate gyrus development GO:0021542 ISO
    cerebellar cortex morphogenesis GO:0021696 ISO
    hippocampus development GO:0021766 ISO
    pyramidal neuron differentiation GO:0021859 ISO
    cerebral cortex development GO:0021987 ISO
    neurogenesis GO:0022008 ISO
    neuron differentiation GO:0030182 ISO
    flagellated sperm motility GO:0030317 ISO
    adult behavior GO:0030534 ISO
    response to tumor necrosis factor GO:0034612 ISO
    locomotory exploration behavior GO:0035641 ISO
    microtubule polymerization GO:0046785 ISO
    forebrain morphogenesis GO:0048853 ISO
    homeostasis of number of cells within a tissue GO:0048873 ISO
    regulation of synapse organization GO:0050807 ISO
    synapse organization GO:0050808 ISO
    neuron apoptotic process GO:0051402 ISO
    motor behavior GO:0061744 ISO
    cellular response to calcium ion GO:0071277 ISO
    organelle transport along microtubule GO:0072384 ISO
    neuron projection arborization GO:0140058 ISO
    response to L-glutamate GO:1902065 ISO
Subcellular Localization
    condensed chromosome GO:0000793 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytosol GO:0005829 ISO
    microtubule GO:0005874 IBA
    microtubule GO:0005874 IEA
    microtubule GO:0005874 ISO
    microtubule GO:0005874 TAS
    axonemal microtubule GO:0005879 ISO
    cytoplasmic microtubule GO:0005881 ISO
    plasma membrane GO:0005886 ISO
    microtubule cytoskeleton GO:0015630 ISO
    motile cilium GO:0031514 IEA
    neuromuscular junction GO:0031594 ISO
    sperm flagellum GO:0036126 ISO
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    myelin sheath GO:0043209 IDA
    membrane raft GO:0045121 IDA
    synapse GO:0045202 ISO
    recycling endosome GO:0055037 ISO
 Experiment description of studies that identified Tuba1a in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 30
MISEV standards
EM
Biophysical techniques
Alix|TSG101|FLOT
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16446100    
Organism Rattus norvegicus
Experiment description Exosomes are released by cultured cortical neurones.
Authors "Faure J, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R"
Journal name MCN
Publication year 2006
Sample Cortical neurones
Sample name Cortical neurones
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Tuba1a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Gja1  
Affinity Capture-MS Rattus norvegicus
2 Map1lc3b 64862
Reconstituted Complex Rattus norvegicus
3 Epn1  
Reconstituted Complex Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
4 HDAC6 10013
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
5 Grm7  
Affinity Capture-MS Rattus norvegicus
Reconstituted Complex Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
Reconstituted Complex Rattus norvegicus
6 Map1lc3a  
Reconstituted Complex Rattus norvegicus
7 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
8 Atxn3  
Affinity Capture-Western Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
9 MLLT10  
Affinity Capture-MS Homo sapiens
10 Dpysl2 25416
Affinity Capture-MS Rattus norvegicus
Affinity Capture-MS Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
11 Rac1 363875
Co-purification Rattus norvegicus
12 Map1a  
Reconstituted Complex Rattus norvegicus
13 ATXN3 4287
Affinity Capture-MS Homo sapiens
14 DYNLL1 8655
Affinity Capture-MS Homo sapiens
15 ABCG1  
Affinity Capture-MS Homo sapiens
16 Epn2  
Affinity Capture-Western Rattus norvegicus
17 Sumo3  
Affinity Capture-MS Rattus norvegicus
18 Park7 117287
Affinity Capture-MS Rattus norvegicus
19 Atxn3  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Tuba1a is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Aggrephagy IEA Reactome
Anchoring of the basal body to the plasma membrane IEA Reactome
Antiviral mechanism by IFN-stimulated genes IEA Reactome
Asparagine N-linked glycosylation IEA Reactome
AURKA Activation by TPX2 IEA Reactome
Autophagy IEA Reactome
Axon guidance IEA Reactome
Carboxyterminal post-translational modifications of tubulin IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Centrosome maturation IEA Reactome
Cilium Assembly IEA Reactome
COPI-dependent Golgi-to-ER retrograde traffic IEA Reactome
COPI-independent Golgi-to-ER retrograde traffic IEA Reactome
COPI-mediated anterograde transport IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
Developmental Biology IEA Reactome
EML4 and NUDC in mitotic spindle formation IEA Reactome
ER to Golgi Anterograde Transport IEA Reactome
Factors involved in megakaryocyte development and platelet production IEA Reactome
G2/M Transition IEA Reactome
Gap junction assembly IEA Reactome
Gap junction trafficking IEA Reactome
Gap junction trafficking and regulation IEA Reactome
Golgi-to-ER retrograde transport IEA Reactome
Hedgehog 'off' state IEA Reactome
Hemostasis IEA Reactome
HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand IEA Reactome
Immune System IEA Reactome
Interferon Signaling IEA Reactome
Intra-Golgi and retrograde Golgi-to-ER traffic IEA Reactome
Intraflagellar transport IEA Reactome
Kinesins IEA Reactome
L1CAM interactions IEA Reactome
Loss of Nlp from mitotic centrosomes IEA Reactome
Loss of proteins required for interphase microtubule organization from the centrosome IEA Reactome
M Phase IEA Reactome
Macroautophagy IEA Reactome
Membrane Trafficking IEA Reactome
Metabolism of proteins IEA Reactome
MHC class II antigen presentation IEA Reactome
Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase IEA Reactome
Nervous system development IEA Reactome
Nuclear Envelope (NE) Reassembly IEA Reactome
Organelle biogenesis and maintenance IEA Reactome
PKR-mediated signaling IEA Reactome
Post-translational protein modification IEA Reactome
Recruitment of mitotic centrosome proteins and complexes IEA Reactome
Recruitment of NuMA to mitotic centrosomes IEA Reactome
Recycling pathway of L1 IEA Reactome
Regulation of PLK1 Activity at G2/M Transition IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
RHO GTPases activate IQGAPs IEA Reactome
Sealing of the nuclear envelope (NE) by ESCRT-III IEA Reactome
Selective autophagy IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by Hedgehog IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
The role of GTSE1 in G2/M progression after G2 checkpoint IEA Reactome
Transport of connexons to the plasma membrane IEA Reactome
Transport to the Golgi and subsequent modification IEA Reactome
Vesicle-mediated transport IEA Reactome





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