Gene description for Dpysl2
Gene name dihydropyrimidinase-like 2
Gene symbol Dpysl2
Other names/aliases Crmp2
TOAD-64
Species Rattus norvegicus
 Database cross references - Dpysl2
ExoCarta ExoCarta_25416
Entrez Gene 25416
UniProt P47942  
 Dpysl2 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Dpysl2
Molecular Function
    protein kinase binding GO:0019901 ISO
    microtubule binding GO:0008017 IEA
    protein binding GO:0005515 IPI
    hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds GO:0016810 IEA
Biological Process
    endocytosis GO:0006897 ISS
    axon guidance GO:0007411 IEA
    response to drug GO:0042493 IEP
    positive regulation of glutamate secretion GO:0014049 IDA
    spinal cord development GO:0021510 IEP
    regulation of axon extension GO:0030516 NAS
    regulation of neuron projection development GO:0010975 ISO
    metabolic process GO:0008152 IEA
    response to amphetamine GO:0001975 IEP
    brain development GO:0007420 IEP
    regulation of neuron differentiation GO:0045664 IMP
    synaptic vesicle transport GO:0048489 IDA
    cytoskeleton organization GO:0007010 ISS
    response to cocaine GO:0042220 IEP
    olfactory bulb development GO:0021772 IEP
Subcellular Localization
    microtubule GO:0005874 ISS
    axon GO:0030424 ISO
    neuron projection GO:0043005 IDA
    dendrite GO:0030425 ISO
    neuronal cell body GO:0043025 ISO
    membrane GO:0016020 ISS
    terminal bouton GO:0043195 IDA
    extracellular exosome GO:0070062 ISO
    myelin sheath GO:0043209 ISO
    growth cone GO:0030426 IDA
    protein complex GO:0043234 IDA
    mitochondrion GO:0005739 ISO
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Dpysl2 in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Dpysl2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Dpysl2 is involved
PathwayEvidenceSource
CRMPs in Sema3A signaling IEA Reactome
Recycling pathway of L1 IEA Reactome





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