Gene description for Dpysl2
Gene name dihydropyrimidinase-like 2
Gene symbol Dpysl2
Other names/aliases Crmp2
TOAD-64
Species Rattus norvegicus
 Database cross references - Dpysl2
ExoCarta ExoCarta_25416
Vesiclepedia VP_25416
Entrez Gene 25416
UniProt P47942  
 Dpysl2 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Dpysl2
Molecular Function
    dihydropyrimidinase activity GO:0004157 IBA
    protein binding GO:0005515 IPI
    hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds GO:0016810 IEA
    hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides GO:0016812 IBA
    protein kinase binding GO:0019901 ISO
    identical protein binding GO:0042802 ISO
Biological Process
    response to amphetamine GO:0001975 IEP
    pyrimidine nucleobase catabolic process GO:0006208 IBA
    endocytosis GO:0006897 ISO
    endocytosis GO:0006897 ISS
    cytoskeleton organization GO:0007010 ISO
    cytoskeleton organization GO:0007010 ISS
    nervous system development GO:0007399 IEA
    response to xenobiotic stimulus GO:0009410 IEP
    regulation of neuron projection development GO:0010975 ISO
    positive regulation of glutamate secretion GO:0014049 IDA
    spinal cord development GO:0021510 IEP
    olfactory bulb development GO:0021772 IEP
    cell differentiation GO:0030154 IEA
    regulation of axon extension GO:0030516 NAS
    response to cocaine GO:0042220 IEP
    regulation of neuron differentiation GO:0045664 IMP
    synaptic vesicle transport GO:0048489 IDA
    regulation of postsynapse assembly GO:0150052 IDA
    regulation of postsynapse assembly GO:0150052 IMP
Subcellular Localization
    cytoplasm GO:0005737 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    microtubule GO:0005874 ISO
    microtubule GO:0005874 ISS
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    postsynaptic density GO:0014069 ISO
    membrane GO:0016020 ISO
    membrane GO:0016020 ISS
    axon GO:0030424 ISO
    dendrite GO:0030425 IDA
    dendrite GO:0030425 ISO
    growth cone GO:0030426 IDA
    protein-containing complex GO:0032991 IDA
    neuronal cell body GO:0043025 IDA
    neuronal cell body GO:0043025 ISO
    terminal bouton GO:0043195 IDA
    myelin sheath GO:0043209 IDA
    synapse GO:0045202 IDA
    synapse GO:0045202 IMP
    Schaffer collateral - CA1 synapse GO:0098685 ISO
    presynapse GO:0098793 IDA
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
 Experiment description of studies that identified Dpysl2 in exosomes
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Dpysl2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tubb3 246118
Affinity Capture-MS Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
Affinity Capture-MS Rattus norvegicus
2 Dpysl2 25416
Affinity Capture-MS Rattus norvegicus
Reconstituted Complex Rattus norvegicus
Affinity Capture-MS Rattus norvegicus
Reconstituted Complex Rattus norvegicus
3 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
4 Tuba1a 64158
Affinity Capture-MS Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
Affinity Capture-MS Rattus norvegicus
5 Katna1  
Affinity Capture-MS Rattus norvegicus
6 Park7 117287
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here