Gene description for Tubb3
Gene name tubulin, beta 3 class III
Gene symbol Tubb3
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Tubb3
ExoCarta ExoCarta_246118
Vesiclepedia VP_246118
Entrez Gene 246118
UniProt Q4QRB4  
 Tubb3 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
 Gene ontology annotations for Tubb3
Molecular Function
    GTPase activity GO:0003924 IEA
    structural constituent of cytoskeleton GO:0005200 IBA
    structural constituent of cytoskeleton GO:0005200 ISO
    structural constituent of cytoskeleton GO:0005200 ISS
    structural constituent of cytoskeleton GO:0005200 NAS
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 ISO
    GTP binding GO:0005525 ISS
    peptide binding GO:0042277 IPI
    metal ion binding GO:0046872 IEA
    netrin receptor binding GO:1990890 ISO
Biological Process
    microtubule cytoskeleton organization GO:0000226 IBA
    microtubule cytoskeleton organization GO:0000226 ISO
    microtubule cytoskeleton organization GO:0000226 ISS
    mitotic cell cycle GO:0000278 IBA
    axon guidance GO:0007411 IBA
    axon guidance GO:0007411 ISO
    axon guidance GO:0007411 ISS
    neuron differentiation GO:0030182 IMP
    netrin-activated signaling pathway GO:0038007 ISO
    netrin-activated signaling pathway GO:0038007 ISS
    dorsal root ganglion development GO:1990791 ISO
    dorsal root ganglion development GO:1990791 ISS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    microtubule GO:0005874 IBA
    microtubule GO:0005874 ISO
    microtubule cytoskeleton GO:0015630 ISO
    microtubule cytoskeleton GO:0015630 ISO
    microtubule cytoskeleton GO:0015630 ISS
    lamellipodium GO:0030027 ISO
    lamellipodium GO:0030027 ISS
    filopodium GO:0030175 ISO
    filopodium GO:0030175 ISS
    axon GO:0030424 IDA
    axon GO:0030424 ISO
    dendrite GO:0030425 ISO
    dendrite GO:0030425 ISS
    growth cone GO:0030426 ISO
    growth cone GO:0030426 ISS
    motile cilium GO:0031514 IEA
    neuronal cell body GO:0043025 ISO
    intercellular bridge GO:0045171 IEA
    intercellular bridge GO:0045171 ISO
    cell periphery GO:0071944 ISO
    mitotic spindle GO:0072686 IEA
    mitotic spindle GO:0072686 ISO
 Experiment description of studies that identified Tubb3 in exosomes
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Tubb3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dpysl2 25416
Affinity Capture-MS Rattus norvegicus
Affinity Capture-MS Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
2 Arfgap1  
Affinity Capture-MS Rattus norvegicus
3 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Tubb3 is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Aggrephagy IEA Reactome
Antiviral mechanism by IFN-stimulated genes IEA Reactome
Asparagine N-linked glycosylation IEA Reactome
Autophagy IEA Reactome
Axon guidance IEA Reactome
Carboxyterminal post-translational modifications of tubulin IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Cilium Assembly IEA Reactome
COPI-dependent Golgi-to-ER retrograde traffic IEA Reactome
COPI-independent Golgi-to-ER retrograde traffic IEA Reactome
COPI-mediated anterograde transport IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
Developmental Biology IEA Reactome
EML4 and NUDC in mitotic spindle formation IEA Reactome
ER to Golgi Anterograde Transport IEA Reactome
Factors involved in megakaryocyte development and platelet production IEA Reactome
G2/M Transition IEA Reactome
Gap junction assembly IEA Reactome
Gap junction trafficking IEA Reactome
Gap junction trafficking and regulation IEA Reactome
Golgi-to-ER retrograde transport IEA Reactome
Hedgehog 'off' state IEA Reactome
Hemostasis IEA Reactome
HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand IEA Reactome
Immune System IEA Reactome
Interferon Signaling IEA Reactome
Intra-Golgi and retrograde Golgi-to-ER traffic IEA Reactome
Intraflagellar transport IEA Reactome
Kinesins IEA Reactome
L1CAM interactions IEA Reactome
M Phase IEA Reactome
Macroautophagy IEA Reactome
Membrane Trafficking IEA Reactome
Metabolism of proteins IEA Reactome
MHC class II antigen presentation IEA Reactome
Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase IEA Reactome
Nervous system development IEA Reactome
Nuclear Envelope (NE) Reassembly IEA Reactome
Organelle biogenesis and maintenance IEA Reactome
PKR-mediated signaling IEA Reactome
Post-translational protein modification IEA Reactome
Recruitment of NuMA to mitotic centrosomes IEA Reactome
Recycling pathway of L1 IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
RHO GTPases activate IQGAPs IEA Reactome
Sealing of the nuclear envelope (NE) by ESCRT-III IEA Reactome
Selective autophagy IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by Hedgehog IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
The role of GTSE1 in G2/M progression after G2 checkpoint IEA Reactome
Transport of connexons to the plasma membrane IEA Reactome
Transport to the Golgi and subsequent modification IEA Reactome
Vesicle-mediated transport IEA Reactome





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