Gene description for Tubb3
Gene name tubulin, beta 3 class III
Gene symbol Tubb3
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Tubb3
ExoCarta ExoCarta_246118
Entrez Gene 246118
UniProt Q4QRB4  
 Tubb3 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
 Gene ontology annotations for Tubb3
Molecular Function
    structural constituent of cytoskeleton GO:0005200 NAS
    peptide binding GO:0042277 IPI
    GTPase activity GO:0003924 IEA
    GTP binding GO:0005525 IEA
Biological Process
    metabolic process GO:0008152 IEA
    mitotic nuclear division GO:0007067 IMP
    protein polymerization GO:0051258 IEA
    neuron differentiation GO:0030182 IMP
    axon guidance GO:0007411 ISS
    microtubule-based process GO:0007017 IEA
Subcellular Localization
    cytoplasm GO:0005737 ISO
    microtubule GO:0005874 ISO
    extracellular exosome GO:0070062 ISO
    intracellular GO:0005622 ISO
    dendrite GO:0030425 ISS
    nucleus GO:0005634 ISO
    microtubule cytoskeleton GO:0015630 ISO
    neuronal cell body GO:0043025 ISO
    axon GO:0030424 ISO
    cell periphery GO:0071944 ISO
 Experiment description of studies that identified Tubb3 in exosomes
1
Experiment ID 225
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD81|CD63
EV Membrane markers
✔
HSPA5
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Tubb3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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