Gene description for Rac1
Gene name ras-related C3 botulinum toxin substrate 1
Gene symbol Rac1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Rac1
ExoCarta ExoCarta_363875
Entrez Gene 363875
UniProt Q6RUV5  
 Rac1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Rac1
Molecular Function
    GTP binding GO:0005525 ISO
    enzyme binding GO:0019899 ISO
    GTPase activity GO:0003924 ISO
    GTP-dependent protein binding GO:0030742 ISO
    protein kinase binding GO:0019901 IPI
    protein binding GO:0005515 IPI
    histone deacetylase binding GO:0042826 IPI
    Rho GDP-dissociation inhibitor binding GO:0051022 IPI
    thioesterase binding GO:0031996 ISO
    Rab GTPase binding GO:0017137 IPI
Biological Process
    cellular response to mechanical stimulus GO:0071260 IDA
    actin filament organization GO:0007015 IMP
    dendrite morphogenesis GO:0048813 ISO
    localization within membrane GO:0051668 ISO
    positive regulation of lamellipodium assembly GO:0010592 ISO
    negative regulation of interleukin-23 production GO:0032707 ISO
    cell motility GO:0048870 ISS
    dendrite development GO:0016358 ISO
    actin cytoskeleton organization GO:0030036 ISO
    positive regulation of stress fiber assembly GO:0051496 ISO
    ruffle assembly GO:0097178 ISO
    endocytosis GO:0006897 ISO
    regulation of fibroblast migration GO:0010762 ISO
    regulation of neuron maturation GO:0014041 ISO
    hyperosmotic response GO:0006972 ISO
    regulation of respiratory burst GO:0060263 ISO
    positive regulation of focal adhesion assembly GO:0051894 ISO
    homeostasis of number of cells within a tissue GO:0048873 ISO
    embryonic olfactory bulb interneuron precursor migration GO:0021831 ISO
    substrate adhesion-dependent cell spreading GO:0034446 ISO
    synaptic transmission, GABAergic GO:0051932 ISO
    metabolic process GO:0008152 IEA
    Wnt signaling pathway, planar cell polarity pathway GO:0060071 ISO
    axon guidance GO:0007411 ISO
    cerebral cortex GABAergic interneuron development GO:0021894 ISO
    positive regulation of DNA replication GO:0045740 IMP
    positive regulation of protein phosphorylation GO:0001934 ISS
    regulation of cell migration GO:0030334 ISS
    phagocytosis, engulfment GO:0006911 ISO
    cerebral cortex radially oriented cell migration GO:0021799 ISO
    cochlea morphogenesis GO:0090103 ISO
    lamellipodium assembly GO:0030032 ISO
    protein localization to plasma membrane GO:0072659 ISO
    bone resorption GO:0045453 IDA
    chemotaxis GO:0006935 IMP
    cell adhesion GO:0007155 ISO
    engulfment of apoptotic cell GO:0043652 ISO
    cytoskeleton organization GO:0007010 ISO
    cell proliferation GO:0008283 IMP
    positive regulation of neutrophil chemotaxis GO:0090023 ISO
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 ISO
    epithelial cell morphogenesis GO:0003382 ISO
    G-protein coupled receptor signaling pathway GO:0007186 ISO
    auditory receptor cell morphogenesis GO:0002093 ISO
    ruffle organization GO:0031529 ISO
    anatomical structure arrangement GO:0048532 ISO
    positive regulation of cell-substrate adhesion GO:0010811 ISO
    positive regulation of phosphatidylinositol 3-kinase activity GO:0043552 ISO
    cell migration GO:0016477 ISO
    regulation of cell morphogenesis GO:0022604 ISO
    dopaminergic neuron differentiation GO:0071542 ISO
    mast cell chemotaxis GO:0002551 IMP
    actin filament polymerization GO:0030041 ISO
    semaphorin-plexin signaling pathway GO:0071526 IMP
    positive regulation of actin filament polymerization GO:0030838 ISO
    small GTPase mediated signal transduction GO:0007264 ISO
    Rac protein signal transduction GO:0016601 TAS
    cell-cell junction organization GO:0045216 ISO
Subcellular Localization
    melanosome GO:0042470 IEA
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    membrane GO:0016020 ISO
    lamellipodium GO:0030027 ISO
    plasma membrane GO:0005886 ISO
    nucleus GO:0005634 ISO
    actin filament GO:0005884 ISO
    trans-Golgi network GO:0005802 ISO
    cytoplasm GO:0005737 ISO
    focal adhesion GO:0005925 ISO
    cytoplasmic membrane-bounded vesicle GO:0016023 ISO
    cell projection GO:0042995 ISO
    cytosol GO:0005829 IDA
    cytoplasmic vesicle GO:0031410 IDA
    phagocytic cup GO:0001891 ISO
    extrinsic component of plasma membrane GO:0019897 ISO
    Golgi membrane GO:0000139 IDA
    ruffle membrane GO:0032587 ISO
    extracellular exosome GO:0070062 ISO
    early endosome membrane GO:0031901 ISO
 Experiment description of studies that identified Rac1 in exosomes
1
Experiment ID 90
ISEV standards
EM
EV Biophysical techniques
HSC70|HSP90|TSG101
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 192
ISEV standards
CEM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
CD63|CD81|FLOT
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Rac1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RGD1309427  
Co-purification Rattus norvegicus
2 Tuba1a 64158
Co-purification Rattus norvegicus
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