Gene description for Dnm2
Gene name dynamin 2
Gene symbol Dnm2
Other names/aliases DYIIAAB
Species Rattus norvegicus
 Database cross references - Dnm2
ExoCarta ExoCarta_25751
Entrez Gene 25751
UniProt P39052  
 Dnm2 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Dnm2
Molecular Function
    GTPase activity GO:0003924 TAS
    protein kinase binding GO:0019901 IPI
    protein complex binding GO:0032403 IDA
    protein binding GO:0005515 IPI
    D2 dopamine receptor binding GO:0031749 IPI
    nitric-oxide synthase binding GO:0050998 IDA
    WW domain binding GO:0050699 IPI
    SH3 domain binding GO:0017124 ISO
    GTP binding GO:0005525 TAS
    phosphatidylinositol 3-kinase regulatory subunit binding GO:0036312 IDA
Biological Process
    G-protein coupled receptor internalization GO:0002031 IMP
    positive regulation of endocytosis GO:0045807 IMP
    regulation of axon extension GO:0030516 IMP
    transferrin transport GO:0033572 ISO
    response to cocaine GO:0042220 IEP
    positive regulation of nitric oxide biosynthetic process GO:0045429 IMP
    receptor internalization GO:0031623 ISO
    positive regulation of phagocytosis GO:0050766 IMP
    regulation of Golgi organization GO:1903358 IMP
    cellular response to nitric oxide GO:0071732 IEP
    cellular response to carbon monoxide GO:0071245 IEP
    macropinocytosis GO:0044351 IMP
    receptor-mediated endocytosis GO:0006898 IMP
    cilium morphogenesis GO:0060271 IMP
    endocytosis GO:0006897 TAS
    positive regulation of lamellipodium assembly GO:0010592 IMP
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IMP
    metabolic process GO:0008152 IEA
    positive regulation of sodium:potassium-exchanging ATPase activity GO:1903408 IMP
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 IMP
    spermatogenesis GO:0007283 IEP
    negative regulation of membrane tubulation GO:1903526 ISO
    cellular response to dopamine GO:1903351 IDA
    response to light stimulus GO:0009416 IEP
    regulation of Rac protein signal transduction GO:0035020 IMP
    cellular response to X-ray GO:0071481 IEP
    Golgi to plasma membrane transport GO:0006893 IMP
    neuron projection morphogenesis GO:0048812 IMP
Subcellular Localization
    focal adhesion GO:0005925 ISO
    extracellular exosome GO:0070062 ISO
    coated pit GO:0005905 IDA
    trans-Golgi network GO:0005802 IDA
    postsynaptic membrane GO:0045211 ISS
    cell junction GO:0030054 IDA
    Golgi membrane GO:0000139 IDA
    centrosome GO:0005813 IDA
    growth cone GO:0030426 IDA
    microtubule GO:0005874 ISO
    protein complex GO:0043234 IDA
    postsynaptic density GO:0014069 IEA
    cytoplasm GO:0005737 ISO
    Golgi apparatus GO:0005794 ISO
    lamellipodium GO:0030027 IDA
    phagocytic cup GO:0001891 IDA
    endosome GO:0005768 IDA
    photoreceptor inner segment GO:0001917 ISO
    nucleus GO:0005634 IDA
    midbody GO:0030496 IEA
    perinuclear region of cytoplasm GO:0048471 IDA
    ruffle membrane GO:0032587 IDA
    plasma membrane GO:0005886 TAS
    cytosol GO:0005829 ISO
    clathrin-coated endocytic vesicle GO:0045334 IDA
 Experiment description of studies that identified Dnm2 in exosomes
1
Experiment ID 90
ISEV standards
EM
EV Biophysical techniques
HSC70|HSP90|TSG101
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Dnm2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DYNLL1 8655
Affinity Capture-MS Homo sapiens
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