Gene description for Pfkp
Gene name phosphofructokinase, platelet
Gene symbol Pfkp
Other names/aliases ATP-PFK
PFK-C
PFK-P
Species Rattus norvegicus
 Database cross references - Pfkp
ExoCarta ExoCarta_60416
Entrez Gene 60416
UniProt P47860  
 Pfkp identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Pfkp
Molecular Function
    metal ion binding GO:0046872 IEA
    protein complex binding GO:0032403 ISO
    ATP binding GO:0005524 IEA
    fructose-6-phosphate binding GO:0070095 IDA
    6-phosphofructokinase activity GO:0003872 ISS
Biological Process
    carbohydrate phosphorylation GO:0046835 ISS
    glycolytic process GO:0006096 IEA
    protein homotetramerization GO:0051289 IDA
    fructose 6-phosphate metabolic process GO:0006002 IEA
    activation of mitophagy in response to mitochondrial depolarization GO:0098779 ISO
    fructose 1,6-bisphosphate metabolic process GO:0030388 IDA
Subcellular Localization
    cytosol GO:0005829 ISO
    cytoplasm GO:0005737 IEA
    extracellular exosome GO:0070062 ISO
    nucleus GO:0005634 ISO
    membrane GO:0016020 ISO
 Experiment description of studies that identified Pfkp in exosomes
1
Experiment ID 96
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Pfkp
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DYNLL1 8655
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Pfkp is involved
No pathways found





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