Gene description for Actg1
Gene name actin, gamma 1
Gene symbol Actg1
Other names/aliases Actg
Species Rattus norvegicus
 Database cross references - Actg1
ExoCarta ExoCarta_287876
Vesiclepedia VP_287876
Entrez Gene 287876
UniProt P63259  
 Actg1 identified in sEVs derived from the following tissue/cell type
Cortical neurones 16446100    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Actg1
Molecular Function
    structural constituent of cytoskeleton GO:0005200 IEA
    structural constituent of cytoskeleton GO:0005200 ISO
    profilin binding GO:0005522 IEA
    profilin binding GO:0005522 ISO
    ATP binding GO:0005524 IEA
    hydrolase activity GO:0016787 IEA
    protein kinase binding GO:0019901 IBA
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    structural constituent of postsynaptic actin cytoskeleton GO:0098973 IBA
    structural constituent of postsynaptic actin cytoskeleton GO:0098973 IDA
Biological Process
    angiogenesis GO:0001525 IEA
    angiogenesis GO:0001525 ISO
    morphogenesis of a polarized epithelium GO:0001738 IEA
    morphogenesis of a polarized epithelium GO:0001738 ISO
    axonogenesis GO:0007409 IBA
    response to mechanical stimulus GO:0009612 IEP
    positive regulation of gene expression GO:0010628 IEA
    positive regulation of gene expression GO:0010628 ISO
    positive regulation of cell migration GO:0030335 IEA
    positive regulation of cell migration GO:0030335 ISO
    sarcomere organization GO:0045214 IEA
    sarcomere organization GO:0045214 ISO
    cell motility GO:0048870 IBA
    regulation of stress fiber assembly GO:0051492 IEA
    regulation of stress fiber assembly GO:0051492 ISO
    response to calcium ion GO:0051592 IEP
    regulation of focal adhesion assembly GO:0051893 IEA
    regulation of focal adhesion assembly GO:0051893 ISO
    platelet aggregation GO:0070527 ISO
    cellular response to type II interferon GO:0071346 IEA
    cellular response to type II interferon GO:0071346 ISO
    positive regulation of wound healing GO:0090303 IEA
    positive regulation of wound healing GO:0090303 ISO
    postsynaptic actin cytoskeleton organization GO:0098974 IEA
    tight junction assembly GO:0120192 IEA
    tight junction assembly GO:0120192 ISO
    regulation of transepithelial transport GO:0150111 IEA
    regulation of transepithelial transport GO:0150111 ISO
    regulation of synaptic vesicle endocytosis GO:1900242 IEA
    regulation of synaptic vesicle endocytosis GO:1900242 ISO
    protein localization to bicellular tight junction GO:1902396 IEA
    protein localization to bicellular tight junction GO:1902396 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IEA
    cytoskeleton GO:0005856 ISS
    actin filament GO:0005884 IBA
    actin filament GO:0005884 ISO
    plasma membrane GO:0005886 ISS
    cell-cell junction GO:0005911 ISO
    focal adhesion GO:0005925 ISS
    actin cytoskeleton GO:0015629 IBA
    actin cytoskeleton GO:0015629 ISO
    membrane GO:0016020 IBA
    myofibril GO:0030016 IEA
    myofibril GO:0030016 ISO
    axon GO:0030424 IBA
    filamentous actin GO:0031941 IEA
    filamentous actin GO:0031941 ISO
    NuA4 histone acetyltransferase complex GO:0035267 IBA
    apical junction complex GO:0043296 IEA
    apical junction complex GO:0043296 ISO
    calyx of Held GO:0044305 IEA
    calyx of Held GO:0044305 ISO
    apical part of cell GO:0045177 ISO
    synapse GO:0045202 IBA
    phagocytic vesicle GO:0045335 IEA
    phagocytic vesicle GO:0045335 ISO
    extracellular exosome GO:0070062 IEA
    extracellular exosome GO:0070062 ISO
    dense body GO:0097433 ISS
    Schaffer collateral - CA1 synapse GO:0098685 IEA
    Schaffer collateral - CA1 synapse GO:0098685 ISO
    postsynaptic actin cytoskeleton GO:0098871 IDA
    presynaptic actin cytoskeleton GO:0099143 IDA
    basal body patch GO:0120220 IEA
    basal body patch GO:0120220 ISO
 Experiment description of studies that identified Actg1 in sEVs
1
Experiment ID 30
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|FLOT
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16446100    
Organism Rattus norvegicus
Experiment description Exosomes are released by cultured cortical neurones.
Authors "Faure J, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R"
Journal name MCN
Publication year 2006
Sample Cortical neurones
Sample name Cortical neurones
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 100
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 101
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Actg1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DYNLL1 8655
Affinity Capture-MS Homo sapiens
2 Dlg4  
Co-fractionation Rattus norvegicus
View the network image/svg+xml
 Pathways in which Actg1 is involved
PathwayEvidenceSource
Adherens junctions interactions IEA Reactome
Axon guidance IEA Reactome
Cell junction organization IEA Reactome
Cell-Cell communication IEA Reactome
Cell-cell junction organization IEA Reactome
Cell-extracellular matrix interactions IEA Reactome
Clathrin-mediated endocytosis IEA Reactome
Developmental Biology IEA Reactome
EPH-Ephrin signaling IEA Reactome
EPHB-mediated forward signaling IEA Reactome
Fcgamma receptor (FCGR) dependent phagocytosis IEA Reactome
Formation of annular gap junctions IEA Reactome
Gap junction degradation IEA Reactome
Gap junction trafficking IEA Reactome
Gap junction trafficking and regulation IEA Reactome
Hemostasis IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Interaction between L1 and Ankyrins IEA Reactome
L1CAM interactions IEA Reactome
MAP2K and MAPK activation IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK1/MAPK3 signaling IEA Reactome
Membrane Trafficking IEA Reactome
Nervous system development IEA Reactome
Platelet activation, signaling and aggregation IEA Reactome
Platelet degranulation IEA Reactome
RAF/MAP kinase cascade IEA Reactome
Recycling pathway of L1 IEA Reactome
Regulation of actin dynamics for phagocytic cup formation IEA Reactome
Response to elevated platelet cytosolic Ca2+ IEA Reactome
RHO GTPase cycle IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
RHO GTPases activate IQGAPs IEA Reactome
RHO GTPases Activate WASPs and WAVEs IEA Reactome
RHOBTB GTPase Cycle IEA Reactome
RHOBTB2 GTPase cycle IEA Reactome
RHOF GTPase cycle IEA Reactome
Signal Transduction IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by VEGF IEA Reactome
VEGFA-VEGFR2 Pathway IEA Reactome
Vesicle-mediated transport IEA Reactome





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