Gene description for MTTP
Gene name microsomal triglyceride transfer protein
Gene symbol MTTP
Other names/aliases ABL
MTP
Species Homo sapiens
 Database cross references - MTTP
ExoCarta ExoCarta_4547
Vesiclepedia VP_4547
Entrez Gene 4547
HGNC 7467
MIM 157147
UniProt P55157  
 MTTP identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
 Gene ontology annotations for MTTP
Molecular Function
    lipid transporter activity GO:0005319 TAS
    protein binding GO:0005515 IPI
    phospholipid transporter activity GO:0005548 IBA
    lipid binding GO:0008289 IEA
    apolipoprotein binding GO:0034185 IEA
    protein-containing complex binding GO:0044877 IEA
    protein heterodimerization activity GO:0046982 IDA
    phospholipid transfer activity GO:0120014 IDA
    phosphatidylcholine transfer activity GO:0120019 IDA
    phosphatidylcholine transfer activity GO:0120019 IMP
    cholesterol transfer activity GO:0120020 IDA
    cholesterol transfer activity GO:0120020 IMP
    triglyceride transfer activity GO:0140344 IDA
    triglyceride transfer activity GO:0140344 IMP
    ceramide 1-phosphate transfer activity GO:1902388 IDA
    phosphatidylethanolamine transfer activity GO:1904121 IDA
Biological Process
    lipid metabolic process GO:0006629 TAS
    triglyceride metabolic process GO:0006641 IEA
    circadian rhythm GO:0007623 IEA
    protein secretion GO:0009306 IDA
    protein secretion GO:0009306 IMP
    phospholipid transport GO:0015914 IDA
    sterol transport GO:0015918 IEA
    triglyceride transport GO:0034197 IDA
    triglyceride transport GO:0034197 IMP
    low-density lipoprotein particle remodeling GO:0034374 IEA
    plasma lipoprotein particle assembly GO:0034377 IDA
    plasma lipoprotein particle assembly GO:0034377 IMP
    chylomicron assembly GO:0034378 TAS
    very-low-density lipoprotein particle assembly GO:0034379 TAS
    lipoprotein metabolic process GO:0042157 IBA
    cholesterol homeostasis GO:0042632 IBA
    lipoprotein transport GO:0042953 IEA
    response to calcium ion GO:0051592 IEA
    establishment of localization in cell GO:0051649 IEA
    intermembrane lipid transfer GO:0120009 IEA
    ceramide 1-phosphate transport GO:1902389 IEA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum lumen GO:0005788 TAS
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
    basolateral plasma membrane GO:0016323 IBA
    brush border membrane GO:0031526 IEA
    microvillus membrane GO:0031528 IEA
    vesicle GO:0031982 IEA
    receptor complex GO:0043235 IDA
 Experiment description of studies that identified MTTP in sEVs
1
Experiment ID 282
MISEV standards
CEM
Biophysical techniques
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
Negative markers
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 283
MISEV standards
CEM
Biophysical techniques
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
Negative markers
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 285
MISEV standards
CEM
Biophysical techniques
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
Negative markers
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
4
Experiment ID 286
MISEV standards
CEM
Biophysical techniques
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
Negative markers
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for MTTP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 P4HB 5034
Affinity Capture-Western Homo sapiens
2 APOB 338
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
3 TMPO 7112
Affinity Capture-MS Homo sapiens
4 PDIA2 64714
Affinity Capture-Western Homo sapiens
5 NUP43 348995
Proximity Label-MS Homo sapiens
6 NF2 4771
Affinity Capture-MS Homo sapiens
7 PRKDC 5591
Cross-Linking-MS (XL-MS) Homo sapiens
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