Experiment description of studies that identified FSIP2 in sEVs

1 Experiment ID 497 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 498 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 260 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1|CD81 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name 1205Lu Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for FSIP2

Protein Interactor ExoCarta ID Identification method PubMed Species 1 HIST2H3A 333932 Cross-Linking-MS (XL-MS) Homo sapiens 2 ANLN 54443 Affinity Capture-MS Homo sapiens 3 ACTR2 10097 Cross-Linking-MS (XL-MS) Homo sapiens 4 ACTB 60 Cross-Linking-MS (XL-MS) Homo sapiens 5 RPS27 6232 Cross-Linking-MS (XL-MS) Homo sapiens 6 RPS27A 6233 Cross-Linking-MS (XL-MS) Homo sapiens 7 HIST1H2BH 8345 Cross-Linking-MS (XL-MS) Homo sapiens 8 HIST1H2BC 8347 Cross-Linking-MS (XL-MS) Homo sapiens 9 LSM7   Cross-Linking-MS (XL-MS) Homo sapiens 10 PTEN 5728 Affinity Capture-MS Homo sapiens 11 API5 8539 Cross-Linking-MS (XL-MS) Homo sapiens 12 PDIA3 2923 Cross-Linking-MS (XL-MS) Homo sapiens 13 FKBP3 2287 Cross-Linking-MS (XL-MS) Homo sapiens 14 CANX 821 Cross-Linking-MS (XL-MS) Homo sapiens 15 NUP43 348995 Proximity Label-MS Homo sapiens 16 TP53 7157 Affinity Capture-MS Homo sapiens 17 AKAP4   Two-hybrid Homo sapiens View the network

Pathways in which FSIP2 is involved

No pathways found