Gene description for Psat1
Gene name phosphoserine aminotransferase 1
Gene symbol Psat1
Other names/aliases D8Ertd814e
EPIP
PSA
Psat
Species Mus musculus
 Database cross references - Psat1
ExoCarta ExoCarta_107272
Entrez Gene 107272
UniProt Q99K85  
 Psat1 identified in exosomes derived from the following tissue/cell type
Mov neuroglial cells 15210972    
Pancreatic cells 19351151    
 Gene ontology annotations for Psat1
Molecular Function
    O-phospho-L-serine:2-oxoglutarate aminotransferase activity GO:0004648 IEA
    transaminase activity GO:0008483 IEA
    pyridoxal phosphate binding GO:0030170 IEA
    catalytic activity GO:0003824 IEA
    transferase activity GO:0016740 IEA
Biological Process
    cellular amino acid biosynthetic process GO:0008652 IEA
    metabolic process GO:0008152 IEA
    L-serine biosynthetic process GO:0006564 IEA
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Psat1 in exosomes
1
Experiment ID 39
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|HSC70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
2
Experiment ID 188
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Psat1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Psat1 is involved
PathwayEvidenceSource
Serine biosynthesis IEA Reactome





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