Gene description for Gnai2
Gene name guanine nucleotide binding protein (G protein), alpha inhibiting 2
Gene symbol Gnai2
Other names/aliases C76432
Galphai2
Gia
Gnai-2
Species Mus musculus
 Database cross references - Gnai2
ExoCarta ExoCarta_14678
Entrez Gene 14678
UniProt P08752  
 Gnai2 identified in exosomes derived from the following tissue/cell type
Basophilic leukemia cells 20424270    
Dendritic cells 10545503    
Embryonic fibroblasts 18494037    
Fibroblasts 23260141    
Mast cells 17486113    
Microglia 16081791    
 Gene ontology annotations for Gnai2
Molecular Function
    GTPase activity GO:0003924 IBA
    nucleotide binding GO:0000166 IEA
    GTP binding GO:0005525 ISA
    guanyl nucleotide binding GO:0019001 IEA
    protein binding GO:0005515 IPI
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    metal ion binding GO:0046872 IEA
    signal transducer activity GO:0004871 IBA
    G-protein coupled receptor binding GO:0001664 IBA
Biological Process
    G-protein coupled receptor signaling pathway GO:0007186 ISO
    negative regulation of synaptic transmission GO:0050805 ISO
    gamma-aminobutyric acid signaling pathway GO:0007214 ISO
    regulation of heart contraction GO:0008016 IMP
    signal transduction GO:0007165 IEA
    positive regulation of cell proliferation GO:0008284 ISO
    cell proliferation GO:0008283 IPI
    activation of MAPKK activity GO:0000186 ISO
    adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway GO:0007193 ISA
    G-protein coupled acetylcholine receptor signaling pathway GO:0007213 IMP
    adenosine receptor signaling pathway GO:0001973 ISO
    cell cycle GO:0007049 IEA
    intracellular signal transduction GO:0035556 ISO
    regulation of calcium ion transport GO:0051924 ISO
    cell division GO:0051301 ISO
    adenylate cyclase-modulating G-protein coupled receptor signaling pathway GO:0007188 IEA
Subcellular Localization
    membrane GO:0016020 ISO
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    extracellular exosome GO:0070062 ISO
    cytoskeleton GO:0005856 IEA
    cytoplasm GO:0005737 ISO
    plasma membrane GO:0005886 ISO
    membrane raft GO:0045121 ISO
    heterotrimeric G-protein complex GO:0005834 IBA
    cytosol GO:0005829 ISO
    extracellular vesicle GO:1903561 ISO
    midbody GO:0030496 ISO
    centrosome GO:0005813 ISO
 Experiment description of studies that identified Gnai2 in exosomes
1
Experiment ID 89
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20424270    
Organism Rattus norvegicus
Experiment description Exosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins.
Authors Subra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B, Silvente-Poirot S, Poirot M, Record M.
Journal name JLR
Publication year 2010
Sample Basophilic leukemia cells
Sample name RBL-2H3
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Gas chromatography
Thin layer chromatography
Mass spectrometry [Qstar XL]
FACS
2
Experiment ID 3
ISEV standards
✔
IEM
EV Biophysical techniques
✔
HSP84|HSC73
EV Cytosolic markers
✔
MHCII|CD9|mac-1
EV Membrane markers
✔
CANX|HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 10545503    
Organism Mus musculus
Experiment description Molecular characterization of dendritic cell-derived exosomes. Selective accumulation of the heat shock protein hsc73.
Authors Thery C, Regnault A, Garin J, Wolfers J, Zitvogel L, Ricciardi-Castagnoli P, Raposo G, Amigorena S.
Journal name JCB
Publication year 1999
Sample Dendritic cells
Sample name D1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
3
Experiment ID 73
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
4
Experiment ID 210
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
6
Experiment ID 14
ISEV standards
✔
EM
EV Biophysical techniques
✔
RAB7|RAB11
EV Cytosolic markers
✔
CD9|CD63|LAMP1|LAMP2
EV Membrane markers
✔
DNM
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
 Protein-protein interactions for Gnai2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here