Gene description for Nans |
Gene name |
N-acetylneuraminic acid synthase (sialic acid synthase) |
Gene symbol |
Nans |
Other names/aliases |
4632418E04Rik BB056474 Sas |
Species |
Mus musculus |
Database cross references - Nans |
ExoCarta |
ExoCarta_94181 |
Vesiclepedia |
VP_94181 |
Entrez Gene |
94181 |
Nans identified in exosomes derived from the following tissue/cell type |
Brain cancer cells
|
19109410
|
Colon cancer cells
|
37309723
|
T-cell lymphoma cells
|
37309723
|
Gene ontology annotations for Nans |
|
Experiment description of studies that identified Nans in exosomes |
1 |
Experiment ID |
26 |
MISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
Alix|GAPDH|HSP70|CD9
|
EV Enriched markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
19109410
|
Organism |
Mus musculus |
Experiment description |
Proteomic and immunologic analyses of brain tumor exosomes. |
Authors |
Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD |
Journal name |
FASEB
|
Publication year |
2008 |
Sample |
Brain cancer cells |
Sample name |
SMA560vIII |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
1.13-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [MALDI TOF/TOF] Western blotting |
|
|
2 |
Experiment ID |
907 |
MISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
|
EV Enriched markers |
✔
HSPA5
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
37309723
|
Organism |
Mus musculus |
Experiment description |
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells |
Authors |
Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S. |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
Colon cancer cells |
Sample name |
C26 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
3 |
Experiment ID |
908 |
MISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
|
EV Enriched markers |
✔
HSPA5
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
Protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
37309723
|
Organism |
Mus musculus |
Experiment description |
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells |
Authors |
Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S. |
Journal name |
Proteomics
|
Publication year |
2023 |
Sample |
T-cell lymphoma cells |
Sample name |
EL4 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
Protein-protein interactions for Nans |
|
Protein Interactor |
ExoCarta ID |
Identification method |
PubMed |
Species |
No interactions are found.
|
|
Pathways in which Nans is involved |
No pathways found
|
|
|