Gene description for Mthfd1
Gene name methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase
Gene symbol Mthfd1
Other names/aliases Dcs
E430024A07Rik
Mthfd
Species Mus musculus
 Database cross references - Mthfd1
ExoCarta ExoCarta_108156
Vesiclepedia VP_108156
Entrez Gene 108156
UniProt Q922D8  
 Mthfd1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Mthfd1
Molecular Function
    ligase activity GO:0016874 IEA
    methylenetetrahydrofolate dehydrogenase (NADP+) activity GO:0004488 IBA
    methylenetetrahydrofolate dehydrogenase (NAD+) activity GO:0004487 IDA
    formate-tetrahydrofolate ligase activity GO:0004329 IMP
    catalytic activity GO:0003824 IEA
    hydrolase activity GO:0016787 IEA
    ATP binding GO:0005524 IEA
    methenyltetrahydrofolate cyclohydrolase activity GO:0004477 IBA
    oxidoreductase activity GO:0016491 IEA
    nucleotide binding GO:0000166 IEA
Biological Process
    heart development GO:0007507 IMP
    purine nucleotide biosynthetic process GO:0006164 IMP
    methionine biosynthetic process GO:0009086 ISO
    metabolic process GO:0008152 IEA
    tetrahydrofolate interconversion GO:0035999 IMP
    methionine metabolic process GO:0006555 IMP
    neural tube closure GO:0001843 IMP
    histidine biosynthetic process GO:0000105 IEA
    serine family amino acid biosynthetic process GO:0009070 ISO
    somite development GO:0061053 IMP
    oxidation-reduction process GO:0055114 IBA
    one-carbon metabolic process GO:0006730 ISO
    10-formyltetrahydrofolate biosynthetic process GO:0009257 IMP
    folic acid-containing compound biosynthetic process GO:0009396 IEA
    cellular amino acid biosynthetic process GO:0008652 IEA
    neutrophil homeostasis GO:0001780 IMP
    transsulfuration GO:0019346 IMP
    serine family amino acid metabolic process GO:0009069 IMP
Subcellular Localization
    mitochondrion GO:0005739 IDA
    cytosol GO:0005829 IBA
    cytoplasm GO:0005737 IEA
    extracellular exosome GO:0070062 ISO
    membrane GO:0016020 ISO
 Experiment description of studies that identified Mthfd1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Mthfd1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Mthfd1 is involved
PathwayEvidenceSource
Metabolism of folate and pterines IEA Reactome





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