Gene description for Uba2
Gene name ubiquitin-like modifier activating enzyme 2
Gene symbol Uba2
Other names/aliases AA986091
Arx
Sae2
UBA1
Ubl1a2
Uble1b
Species Mus musculus
 Database cross references - Uba2
ExoCarta ExoCarta_50995
Vesiclepedia VP_50995
Entrez Gene 50995
UniProt Q9Z1F9  
 Uba2 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Uba2
Molecular Function
    protein heterodimerization activity GO:0046982 ISO
    tubulin-glutamic acid ligase activity GO:0070740 IEA
    protein-glutamic acid ligase activity GO:0070739 IEA
    SUMO activating enzyme activity GO:0019948 ISO
    ligase activity GO:0016874 IEA
    protein-glycine ligase activity GO:0070735 IEA
    protein binding GO:0005515 IPI
    small protein activating enzyme activity GO:0008641 IEA
    ribosomal S6-glutamic acid ligase activity GO:0018169 IEA
    tubulin-glycine ligase activity GO:0070738 IEA
    nucleotide binding GO:0000166 IEA
    protein-glycine ligase activity, elongating GO:0070737 IEA
    transcription factor binding GO:0008134 IPI
    coenzyme F420-0 gamma-glutamyl ligase activity GO:0043773 IEA
    protein-glycine ligase activity, initiating GO:0070736 IEA
    ATP binding GO:0005524 IEA
    UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity GO:0008766 IEA
    coenzyme F420-2 alpha-glutamyl ligase activity GO:0043774 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    protein sumoylation GO:0016925 ISO
    cellular protein modification process GO:0006464 IEA
Subcellular Localization
    cytoplasm GO:0005737 IEA
    nucleus GO:0005634 ISO
    SUMO activating enzyme complex GO:0031510 ISO
    cytosol GO:0005829 IBA
    nucleoplasm GO:0005654 ISO
 Experiment description of studies that identified Uba2 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Uba2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Uba2 is involved
PathwayEvidenceSource
SUMO is conjugated to E1 (UBA2:SAE1) IEA Reactome
SUMO is transferred from E1 to E2 (UBE2I, UBC9) IEA Reactome





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