Gene description for PHTF1
Gene name putative homeodomain transcription factor 1
Gene symbol PHTF1
Other names/aliases PHTF
Species Homo sapiens
 Database cross references - PHTF1
ExoCarta ExoCarta_10745
Entrez Gene 10745
HGNC 8939
MIM 604950
UniProt Q9UMS5  
 PHTF1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for PHTF1
Molecular Function
    DNA binding GO:0003677 IEA
    sequence-specific DNA binding transcription factor activity GO:0003700 TAS
Biological Process
    regulation of transcription, DNA-templated GO:0006355 IEA
    transcription, DNA-templated GO:0006351 IEA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IEA
    nucleus GO:0005634 IDA
    cis-Golgi network GO:0005801 IEA
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified PHTF1 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for PHTF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which PHTF1 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here