Gene description for Cyb5r3
Gene name cytochrome b5 reductase 3
Gene symbol Cyb5r3
Other names/aliases 0610016L08Rik
2500002N19Rik
C85115
Dia-1
Dia1
WU:AL591952.1-001
WU:AL591952.1-002
WU:AL591952.1-003
WU:Cyb5r3
Species Mus musculus
 Database cross references - Cyb5r3
ExoCarta ExoCarta_109754
Vesiclepedia VP_109754
Entrez Gene 109754
UniProt Q9DCN2  
 Cyb5r3 identified in sEVs derived from the following tissue/cell type
Pancreatic cells 19351151    
 Gene ontology annotations for Cyb5r3
Molecular Function
    cytochrome-b5 reductase activity, acting on NAD(P)H GO:0004128 ISO
    cytochrome-b5 reductase activity, acting on NAD(P)H GO:0004128 ISS
    protein binding GO:0005515 IPI
    AMP binding GO:0016208 ISO
    ADP binding GO:0043531 ISO
    nitrite reductase (NO-forming) activity GO:0050421 ISO
    flavin adenine dinucleotide binding GO:0050660 ISO
    NAD binding GO:0051287 ISO
    FAD binding GO:0071949 IBA
    FAD binding GO:0071949 ISO
    FAD binding GO:0071949 ISS
Biological Process
    cholesterol biosynthetic process GO:0006695 IEA
    nitric oxide biosynthetic process GO:0006809 ISO
Subcellular Localization
    mitochondrion GO:0005739 IBA
    mitochondrial outer membrane GO:0005741 ISO
    mitochondrial outer membrane GO:0005741 ISS
    mitochondrial inner membrane GO:0005743 HDA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum membrane GO:0005789 ISO
    endoplasmic reticulum membrane GO:0005789 ISO
    endoplasmic reticulum membrane GO:0005789 ISS
    endoplasmic reticulum membrane GO:0005789 ISS
    lipid droplet GO:0005811 ISO
    cytosol GO:0005829 ISO
    mitochondrial membrane GO:0031966 ISO
    nitric-oxide synthase complex GO:1903958 ISO
 Experiment description of studies that identified Cyb5r3 in sEVs
1
Experiment ID 188
MISEV standards
EM
Biophysical techniques
GAPDH|UCHL1|HSP90
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Cyb5r3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Bnip3  
Cross-Linking-MS (XL-MS) Mus musculus
2 Mettl21e  
Affinity Capture-MS Mus musculus
3 Cyb5r3 109754
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
4 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml



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