Gene description for Pi4k2a
Gene name phosphatidylinositol 4-kinase type 2 alpha
Gene symbol Pi4k2a
Other names/aliases Pi4KII
Species Rattus norvegicus
 Database cross references - Pi4k2a
ExoCarta ExoCarta_114554
Vesiclepedia VP_114554
Entrez Gene 114554
UniProt Q99M64  
 Pi4k2a identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Pi4k2a
Molecular Function
    1-phosphatidylinositol 4-kinase activity GO:0004430 IBA
    1-phosphatidylinositol 4-kinase activity GO:0004430 IDA
    1-phosphatidylinositol 4-kinase activity GO:0004430 IEA
    1-phosphatidylinositol 4-kinase activity GO:0004430 ISO
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    ATP binding GO:0005524 ISS
    AP-3 adaptor complex binding GO:0035651 IEA
    AP-3 adaptor complex binding GO:0035651 ISO
    AP-3 adaptor complex binding GO:0035651 ISS
    protein-containing complex binding GO:0044877 IDA
Biological Process
    basophil degranulation GO:0002561 IMP
    phosphatidylinositol biosynthetic process GO:0006661 ISO
    phosphatidylinositol biosynthetic process GO:0006661 ISS
    Golgi organization GO:0007030 IBA
    endosome organization GO:0007032 IBA
    phosphatidylinositol phosphate biosynthetic process GO:0046854 IBA
    phosphatidylinositol phosphate biosynthetic process GO:0046854 IEA
    phosphatidylinositol phosphate biosynthetic process GO:0046854 ISO
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    Golgi membrane GO:0000139 ISO
    mitochondrion GO:0005739 IEA
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISS
    endosome GO:0005768 IBA
    endosome GO:0005768 IDA
    trans-Golgi network GO:0005802 IBA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    endosome membrane GO:0010008 ISO
    membrane GO:0016020 ISO
    membrane GO:0016020 ISS
    dendrite GO:0030425 IEA
    dendrite GO:0030425 ISO
    dendrite GO:0030425 ISS
    synaptic vesicle membrane GO:0030672 IDA
    BLOC-1 complex GO:0031083 IEA
    BLOC-1 complex GO:0031083 ISO
    BLOC-1 complex GO:0031083 ISS
    cytoplasmic vesicle GO:0031410 IDA
    early endosome membrane GO:0031901 IDA
    early endosome membrane GO:0031901 IEA
    early endosome membrane GO:0031901 ISO
    protein-containing complex GO:0032991 IDA
    growing cell tip GO:0035838 IDA
    presynaptic membrane GO:0042734 IEA
    presynaptic membrane GO:0042734 ISO
    presynaptic membrane GO:0042734 ISS
    neuron projection GO:0043005 IDA
    neuron projection GO:0043005 ISO
    neuronal cell body GO:0043025 IDA
    neuronal cell body GO:0043025 ISO
    neuronal cell body GO:0043025 ISS
    perikaryon GO:0043204 IDA
    perikaryon GO:0043204 IEA
    membrane raft GO:0045121 IEA
    membrane raft GO:0045121 ISO
    presynaptic active zone GO:0048786 IEA
    presynaptic active zone GO:0048786 ISO
    exocytic vesicle GO:0070382 IDA
    glutamatergic synapse GO:0098978 IEA
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Pi4k2a in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Pi4k2a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Itch 311567
Affinity Capture-Western Rattus norvegicus
View the network image/svg+xml



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