Gene description for Pi4k2a
Gene name phosphatidylinositol 4-kinase type 2 alpha
Gene symbol Pi4k2a
Other names/aliases Pi4KII
Species Rattus norvegicus
 Database cross references - Pi4k2a
ExoCarta ExoCarta_114554
Entrez Gene 114554
UniProt Q99M64  
 Pi4k2a identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Pi4k2a
Molecular Function
    protein complex binding GO:0032403 IDA
    1-phosphatidylinositol 4-kinase activity GO:0004430 ISO
    ATP binding GO:0005524 ISS
    AP-3 adaptor complex binding GO:0035651 ISS
Biological Process
    phosphatidylinositol biosynthetic process GO:0006661 ISS
    phosphatidylinositol phosphorylation GO:0046854 ISO
    basophil degranulation GO:0002561 IMP
    activation of mitophagy in response to mitochondrial depolarization GO:0098779 ISO
Subcellular Localization
    integral component of plasma membrane GO:0005887 ISS
    cytoplasmic vesicle GO:0031410 IDA
    lysosomal membrane GO:0005765 ISO
    synaptic vesicle membrane GO:0030672 IDA
    protein complex GO:0043234 IDA
    membrane raft GO:0045121 ISO
    neuron projection GO:0043005 ISO
    presynaptic membrane GO:0042734 ISS
    BLOC-1 complex GO:0031083 ISS
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 ISO
    intracellular membrane-bounded organelle GO:0043231 IDA
    early endosome membrane GO:0031901 IDA
    intrinsic component of membrane GO:0031224 ISS
    host cell presynaptic membrane GO:0044231 ISS
    neuronal cell body GO:0043025 ISS
    mitochondrion GO:0005739 ISS
    growing cell tip GO:0035838 IDA
    exocytic vesicle GO:0070382 IDA
    dendrite GO:0030425 ISS
    cell junction GO:0030054 IEA
    Golgi apparatus GO:0005794 IEA
    endosome GO:0005768 IDA
    perikaryon GO:0043204 IDA
 Experiment description of studies that identified Pi4k2a in exosomes
1
Experiment ID 90
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSC70|HSP90|TSG101
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Pi4k2a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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