ExoCarta: Gene summary

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Gene description for Itch
Gene name	itchy E3 ubiquitin protein ligase
Gene symbol	Itch
Other names/aliases	-
Species	Rattus norvegicus
Database cross references - Itch
ExoCarta	ExoCarta_311567
Entrez Gene	311567
Itch identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Itch

Molecular Function
	ubiquitin-protein transferase activity	GO:0004842	ISO
	ubiquitin protein ligase activity	GO:0061630	ISO
	ribonucleoprotein complex binding	GO:0043021	ISO
	protein binding	GO:0005515	IPI
	ligase activity	GO:0016874	ISO
	CXCR chemokine receptor binding	GO:0045236	ISO
Biological Process
	protein K29-linked ubiquitination	GO:0035519	ISO
	regulation of protein deubiquitination	GO:0090085	ISO
	negative regulation of NF-kappaB transcription factor activity	GO:0032088	ISO
	positive regulation of T cell anergy	GO:0002669	ISO
	ubiquitin-dependent protein catabolic process	GO:0006511	ISO
	protein polyubiquitination	GO:0000209	ISO
	negative regulation of JNK cascade	GO:0046329	ISO
	positive regulation of protein catabolic process	GO:0045732	ISO
	protein K63-linked ubiquitination	GO:0070534	ISO
	negative regulation of defense response to virus	GO:0050687	ISO
	protein K48-linked ubiquitination	GO:0070936	ISO
	protein ubiquitination involved in ubiquitin-dependent protein catabolic process	GO:0042787	ISO
	negative regulation of alpha-beta T cell proliferation	GO:0046642	ISO
	protein ubiquitination	GO:0016567	ISO
	negative regulation of apoptotic process	GO:0043066	ISO
Subcellular Localization
	membrane	GO:0016020	ISO
	nucleus	GO:0005634	ISO
	cytoplasmic vesicle	GO:0031410	ISO
	extracellular exosome	GO:0070062	ISO
	cytoplasm	GO:0005737	ISO
	cell cortex	GO:0005938	ISO
	plasma membrane	GO:0005886	ISO

Experiment description of studies that identified Itch in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D2 Rep 2

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting