Gene description for Itch
Gene name itchy E3 ubiquitin protein ligase
Gene symbol Itch
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Itch
ExoCarta ExoCarta_311567
Vesiclepedia VP_311567
Entrez Gene 311567
 Itch identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Itch
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 ISO
    ligase activity GO:0016874 ISO
    ubiquitin-ubiquitin ligase activity GO:0034450 ISO
    ribonucleoprotein complex binding GO:0043021 ISO
    ubiquitin-like protein ligase binding GO:0044389 ISO
    CXCR chemokine receptor binding GO:0045236 ISO
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase activity GO:0061630 ISO
    arrestin family protein binding GO:1990763 ISO
Biological Process
    protein polyubiquitination GO:0000209 ISO
    positive regulation of T cell anergy GO:0002669 ISO
    T cell anergy GO:0002870 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 ISO
    protein monoubiquitination GO:0006513 ISO
    protein ubiquitination GO:0016567 ISO
    protein catabolic process GO:0030163 ISO
    receptor internalization GO:0031623 ISO
    negative regulation of NF-kappaB transcription factor activity GO:0032088 ISO
    protein K29-linked ubiquitination GO:0035519 ISO
    CD4-positive, alpha-beta T cell proliferation GO:0035739 ISO
    negative regulation of cytoplasmic pattern recognition receptor signaling pathway GO:0039532 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 ISO
    positive regulation of protein catabolic process GO:0045732 ISO
    negative regulation of JNK cascade GO:0046329 ISO
    negative regulation of defense response to virus GO:0050687 ISO
    protein autoubiquitination GO:0051865 ISO
    protein K63-linked ubiquitination GO:0070534 ISO
    protein K48-linked ubiquitination GO:0070936 ISO
    regulation of protein deubiquitination GO:0090085 ISO
    protein branched polyubiquitination GO:0141198 ISO
    negative regulation of CD4-positive, alpha-beta T cell proliferation GO:2000562 ISO
    positive regulation of receptor catabolic process GO:2000646 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    early endosome GO:0005769 ISO
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISO
    cell cortex GO:0005938 ISO
    membrane GO:0016020 ISO
    cytoplasmic vesicle GO:0031410 ISO
    protein-containing complex GO:0032991 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Itch in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Itch
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ocln  
Affinity Capture-Western Rattus norvegicus
2 Pi4k2a  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
3 Pi4k2a 114554
Affinity Capture-Western Rattus norvegicus
View the network image/svg+xml
 Pathways in which Itch is involved
No pathways found





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