Gene description for Ak4
Gene name adenylate kinase 4
Gene symbol Ak4
Other names/aliases AK 4
Ak-3
Ak-4
Ak3
Ak3l1
D4Ertd274e
Species Mus musculus
 Database cross references - Ak4
ExoCarta ExoCarta_11639
Vesiclepedia VP_11639
Entrez Gene 11639
UniProt Q9WUR9  
 Ak4 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Ak4
Molecular Function
    adenylate kinase activity GO:0004017 IEA
    adenylate kinase activity GO:0004017 ISO
    nucleoside diphosphate kinase activity GO:0004550 IBA
    nucleoside diphosphate kinase activity GO:0004550 ISO
    nucleoside diphosphate kinase activity GO:0004550 ISS
    ATP binding GO:0005524 IEA
    GTP binding GO:0005525 IEA
    CMP kinase activity GO:0036430 IEA
    dCMP kinase activity GO:0036431 IEA
    nucleoside triphosphate adenylate kinase activity GO:0046899 IEA
    nucleoside triphosphate adenylate kinase activity GO:0046899 ISO
    (deoxy)adenylate kinase activity GO:0047506 IEA
Biological Process
    regulation of oxidative phosphorylation GO:0002082 ISO
    regulation of oxidative phosphorylation GO:0002082 ISS
    ADP biosynthetic process GO:0006172 IEA
    nucleoside triphosphate biosynthetic process GO:0009142 IBA
    ribonucleoside diphosphate biosynthetic process GO:0009188 ISO
    ribonucleoside diphosphate biosynthetic process GO:0009188 ISS
    AMP metabolic process GO:0046033 IBA
    AMP metabolic process GO:0046033 IEA
    AMP metabolic process GO:0046033 ISO
    ATP metabolic process GO:0046034 IEA
    ATP metabolic process GO:0046034 ISO
    GTP metabolic process GO:0046039 IEA
    GTP metabolic process GO:0046039 ISO
    nucleoside monophosphate phosphorylation GO:0046940 IEA
    cellular response to hypoxia GO:0071456 ISO
    cellular response to hypoxia GO:0071456 ISO
    cellular response to hypoxia GO:0071456 ISS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 ISO
    mitochondrial matrix GO:0005759 IBA
    mitochondrial matrix GO:0005759 IDA
 Experiment description of studies that identified Ak4 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Ak4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ak4 11639
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
2 Ubc  
Reconstituted Complex Mus musculus
3 Dlst  
Cross-Linking-MS (XL-MS) Mus musculus
View the network image/svg+xml
 Pathways in which Ak4 is involved
PathwayEvidenceSource
Interconversion of nucleotide di- and triphosphates IEA Reactome
Metabolism IEA Reactome
Metabolism of nucleotides IEA Reactome





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