Gene description for H3f3b
Gene name H3 histone, family 3B
Gene symbol H3f3b
Other names/aliases H3.3B
Species Rattus norvegicus
 Database cross references - H3f3b
ExoCarta ExoCarta_117056
Entrez Gene 117056
UniProt P84245  
 H3f3b identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for H3f3b
Molecular Function
    DNA binding GO:0003677 IEA
    molecular_function GO:0003674 ND
    histone binding GO:0042393 ISO
    protein heterodimerization activity GO:0046982 IEA
    RNA polymerase II distal enhancer sequence-specific DNA binding GO:0000980 ISO
    nucleosomal DNA binding GO:0031492 ISO
    RNA polymerase II core promoter sequence-specific DNA binding GO:0000979 ISO
Biological Process
    muscle cell differentiation GO:0042692 ISO
    nucleus organization GO:0006997 ISO
    osteoblast differentiation GO:0001649 ISO
    positive regulation of cell growth GO:0030307 ISO
    nucleosome assembly GO:0006334 ISO
    brain development GO:0007420 IEP
    response to hormone GO:0009725 IEP
    negative regulation of chromosome condensation GO:1902340 ISO
    regulation of centromere complex assembly GO:0090230 ISO
    DNA replication-independent nucleosome assembly GO:0006336 ISO
Subcellular Localization
    nuclear chromosome GO:0000228 ISO
    protein complex GO:0043234 ISO
    nucleosome GO:0000786 ISO
    cellular_component GO:0005575 ND
    extracellular exosome GO:0070062 ISO
    nuclear nucleosome GO:0000788 ISO
    nucleus GO:0005634 ISO
 Experiment description of studies that identified H3f3b in exosomes
1
Experiment ID 225
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 92
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|GAPDH
EV Cytosolic markers
✔
CD9|CD151
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
3
Experiment ID 93
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|GAPDH
EV Cytosolic markers
✔
CD9|CD151
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for H3f3b
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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