Gene description for H3f3b
Gene name H3 histone, family 3B
Gene symbol H3f3b
Other names/aliases H3.3B
Species Rattus norvegicus
 Database cross references - H3f3b
ExoCarta ExoCarta_117056
Vesiclepedia VP_117056
Entrez Gene 117056
UniProt P84245  
 H3f3b identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for H3f3b
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 ISO
    RNA polymerase II core promoter sequence-specific DNA binding GO:0000979 ISO
    structural constituent of chromatin GO:0030527 ISO
    nucleosomal DNA binding GO:0031492 ISO
    protein heterodimerization activity GO:0046982 IEA
Biological Process
    osteoblast differentiation GO:0001649 ISO
    nucleosome assembly GO:0006334 ISO
    nucleus organization GO:0006997 ISO
    spermatogenesis GO:0007283 ISO
    spermatid development GO:0007286 ISO
    single fertilization GO:0007338 ISO
    embryo implantation GO:0007566 ISO
    cell population proliferation GO:0008283 ISO
    male gonad development GO:0008584 ISO
    response to hormone GO:0009725 IEP
    positive regulation of cell growth GO:0030307 ISO
    pericentric heterochromatin formation GO:0031508 ISO
    subtelomeric heterochromatin formation GO:0031509 ISO
    multicellular organism growth GO:0035264 ISO
    muscle cell differentiation GO:0042692 ISO
    oogenesis GO:0048477 ISO
    regulation of centromere complex assembly GO:0090230 ISO
    negative regulation of chromosome condensation GO:1902340 ISO
Subcellular Localization
    chromosome, telomeric region GO:0000781 ISO
    nucleosome GO:0000786 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    protein-containing complex GO:0032991 ISO
 Experiment description of studies that identified H3f3b in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 92
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|GAPDH|CD9|CD151
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
3
Experiment ID 93
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|GAPDH|CD9|CD151
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for H3f3b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 H3f3b 117056
Co-localization Rattus norvegicus
Co-localization Rattus norvegicus
2 Parp1  
Co-localization Rattus norvegicus
View the network image/svg+xml
 Pathways in which H3f3b is involved
PathwayEvidenceSource
Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 IEA Reactome
Assembly of the ORC complex at the origin of replication IEA Reactome
Assembly of the pre-replicative complex IEA Reactome
B-WICH complex positively regulates rRNA expression IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Cellular Senescence IEA Reactome
DNA Replication IEA Reactome
DNA Replication Pre-Initiation IEA Reactome
Epigenetic regulation by WDR5-containing histone modifying complexes IEA Reactome
Epigenetic regulation of adipogenesis genes by MLL3 and MLL4 complexes IEA Reactome
Epigenetic regulation of gene expression IEA Reactome
Epigenetic regulation of gene expression by MLL3 and MLL4 complexes IEA Reactome
ESR-mediated signaling IEA Reactome
Estrogen-dependent gene expression IEA Reactome
Factors involved in megakaryocyte development and platelet production IEA Reactome
Gene expression (Transcription) IEA Reactome
Gene Silencing by RNA IEA Reactome
Generic Transcription Pathway IEA Reactome
Hemostasis IEA Reactome
MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis IEA Reactome
Negative epigenetic regulation of rRNA expression IEA Reactome
NoRC negatively regulates rRNA expression IEA Reactome
Oxidative Stress Induced Senescence IEA Reactome
Positive epigenetic regulation of rRNA expression IEA Reactome
PRC2 methylates histones and DNA IEA Reactome
Regulation of endogenous retroelements IEA Reactome
Regulation of endogenous retroelements by KRAB-ZFP proteins IEA Reactome
Regulation of endogenous retroelements by the Human Silencing Hub (HUSH) complex IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases activate PKNs IEA Reactome
RNA Polymerase I Promoter Clearance IEA Reactome
RNA Polymerase I Promoter Escape IEA Reactome
RNA Polymerase I Promoter Opening IEA Reactome
RNA Polymerase I Transcription IEA Reactome
RNA Polymerase II Transcription IEA Reactome
RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function IEA Reactome
Senescence-Associated Secretory Phenotype (SASP) IEA Reactome
Signal Transduction IEA Reactome
Signaling by Nuclear Receptors IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
SIRT1 negatively regulates rRNA expression IEA Reactome
Transcriptional regulation by RUNX1 IEA Reactome
Transcriptional regulation by small RNAs IEA Reactome





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