Experiment description of studies that identified Hnrnpk in exosomes |
1 |
Experiment ID |
225 |
MISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
GAPDH|CD63
|
EV Enriched markers |
✘
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25998041
|
Organism |
Rattus norvegicus |
Experiment description |
Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats. |
Authors |
Lee JE, Moon PG, Lee IK, Baek MC |
Journal name |
Protein J
|
Publication year |
2015 |
Sample |
Adipocytes |
Sample name |
Adipocytes |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
2 |
Experiment ID |
94 |
MISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP90|HSC70|MHCI
|
EV Enriched markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [Orbitrap]
|
PubMed ID |
21828046
|
Organism |
Rattus norvegicus |
Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors |
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. |
Journal name |
JBC
|
Publication year |
2011 |
Sample |
Reticulocytes |
Sample name |
Reticulocytes - D4 Rep 1 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.16-1.21 g/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
3 |
Experiment ID |
95 |
MISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP90|HSC70|MHCI
|
EV Enriched markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [Orbitrap]
|
PubMed ID |
21828046
|
Organism |
Rattus norvegicus |
Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors |
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. |
Journal name |
JBC
|
Publication year |
2011 |
Sample |
Reticulocytes |
Sample name |
Reticulocytes - D7 Rep 1 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.13-1.25 g/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
4 |
Experiment ID |
96 |
MISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP90|HSC70|MHCI
|
EV Enriched markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [Orbitrap]
|
PubMed ID |
21828046
|
Organism |
Rattus norvegicus |
Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors |
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. |
Journal name |
JBC
|
Publication year |
2011 |
Sample |
Reticulocytes |
Sample name |
Reticulocytes - D2 Rep 2 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.16-1.21 g/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
5 |
Experiment ID |
97 |
MISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP90|HSC70|MHCI
|
EV Enriched markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [Orbitrap]
|
PubMed ID |
21828046
|
Organism |
Rattus norvegicus |
Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors |
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. |
Journal name |
JBC
|
Publication year |
2011 |
Sample |
Reticulocytes |
Sample name |
Reticulocytes - D4 Rep 2 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.16-1.21 g/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
6 |
Experiment ID |
98 |
MISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|HSP90|HSC70|MHCI
|
EV Enriched markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [Orbitrap]
|
PubMed ID |
21828046
|
Organism |
Rattus norvegicus |
Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors |
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. |
Journal name |
JBC
|
Publication year |
2011 |
Sample |
Reticulocytes |
Sample name |
Reticulocytes - D7 Rep 2 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.13-1.25 g/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|