Gene description for Hnrnpk
Gene name heterogeneous nuclear ribonucleoprotein K
Gene symbol Hnrnpk
Other names/aliases Csbp
Hnrpk
Species Rattus norvegicus
 Database cross references - Hnrnpk
ExoCarta ExoCarta_117282
Entrez Gene 117282
UniProt P61980  
 Hnrnpk identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Hnrnpk
Molecular Function
    mRNA 3'-UTR binding GO:0003730 IDA
    protein binding GO:0005515 IPI
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 ISO
    ATPase binding GO:0051117 IPI
    RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001077 ISO
    poly(A) RNA binding GO:0044822 ISO
    RNA binding GO:0003723 ISO
    core promoter proximal region DNA binding GO:0001159 IDA
    single-stranded DNA binding GO:0003697 IDA
Biological Process
    positive regulation of neuron projection development GO:0010976 IMP
    RNA splicing GO:0008380 IEA
    positive regulation of low-density lipoprotein particle receptor biosynthetic process GO:0045716 ISO
    transcription, DNA-templated GO:0006351 IEA
    peripheral nervous system development GO:0007422 IEP
    positive regulation of dendritic spine development GO:0060999 IMP
    positive regulation of dendrite extension GO:1903861 IMP
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IMP
    negative regulation of branching morphogenesis of a nerve GO:2000173 IMP
    positive regulation of long-term synaptic potentiation GO:1900273 IMP
    negative regulation of mRNA splicing, via spliceosome GO:0048025 ISO
    liver development GO:0001889 IEP
    cerebral cortex development GO:0021987 IEP
    central nervous system development GO:0007417 IEP
    positive regulation of protein localization to cell surface GO:2000010 IMP
    kidney development GO:0001822 IEP
    thymus development GO:0048538 IEP
    regulation of low-density lipoprotein particle clearance GO:0010988 ISO
    camera-type eye development GO:0043010 IEP
    hippocampus development GO:0021766 IEP
    negative regulation of apoptotic process GO:0043066 IMP
    positive regulation of synaptic transmission GO:0050806 IMP
    cellular response to organic cyclic compound GO:0071407 IDA
    positive regulation of synapse maturation GO:0090129 IMP
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    ovarian follicle development GO:0001541 IMP
    lung development GO:0030324 IEP
    cerebellum development GO:0021549 IEP
    cellular response to glucose stimulus GO:0071333 IEP
    regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:1902165 ISO
    mRNA processing GO:0006397 IEA
    regulation of lipid transport by positive regulation of transcription from RNA polymerase II promoter GO:0072369 ISO
    cellular response to amino acid stimulus GO:0071230 IEP
    regulation of transcription from RNA polymerase II promoter GO:0006357 IMP
    positive regulation of receptor-mediated endocytosis GO:0048260 ISO
    positive regulation of RNA splicing GO:0033120 IMP
    regulation of transcription, DNA-templated GO:0006355 IEA
    cellular response to insulin stimulus GO:0032869 IEP
Subcellular Localization
    axon terminus GO:0043679 IDA
    spliceosomal complex GO:0005681 IEA
    cell projection GO:0042995 IEA
    nucleoplasm GO:0005654 IEA
    catalytic step 2 spliceosome GO:0071013 ISO
    cytosol GO:0005829 IDA
    membrane GO:0016020 ISO
    extracellular exosome GO:0070062 ISO
    cell junction GO:0030054 IEA
    dendritic spine GO:0043197 IDA
    focal adhesion GO:0005925 ISO
    postsynaptic density GO:0014069 IDA
    nuclear chromatin GO:0000790 ISO
    cytoplasm GO:0005737 IEA
    podosome GO:0002102 IEA
    mitochondrion GO:0005739 IDA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified Hnrnpk in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 94
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Hnrnpk
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Hnrnpk is involved
PathwayEvidenceSource
mRNA Splicing - Major Pathway IEA Reactome





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