Gene description for Sept2
Gene name septin 2
Gene symbol Sept2
Other names/aliases Nedd5
Vesp11
Species Rattus norvegicus
 Database cross references - Sept2
ExoCarta ExoCarta_117515
Vesiclepedia VP_117515
Entrez Gene 117515
UniProt Q91Y81  
 Sept2 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Sept2
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 TAS
    GTP binding GO:0005525 IEA
    enzyme regulator activity GO:0030234 IEA
    enzyme regulator activity GO:0030234 ISO
    identical protein binding GO:0042802 ISO
    molecular adaptor activity GO:0060090 IBA
Biological Process
    regulation of L-glutamate import across plasma membrane GO:0002036 IEA
    regulation of L-glutamate import across plasma membrane GO:0002036 ISO
    smoothened signaling pathway GO:0007224 IEA
    smoothened signaling pathway GO:0007224 ISO
    smoothened signaling pathway GO:0007224 ISS
    spermatogenesis GO:0007283 IEA
    protein localization GO:0008104 IBA
    neuron projection development GO:0031175 IMP
    regulation of protein localization GO:0032880 IEA
    regulation of protein localization GO:0032880 ISO
    cell division GO:0051301 IEA
    cilium assembly GO:0060271 IBA
    cilium assembly GO:0060271 IEA
    cilium assembly GO:0060271 ISO
    cilium assembly GO:0060271 ISS
    cytoskeleton-dependent cytokinesis GO:0061640 IBA
Subcellular Localization
    exocyst GO:0000145 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    spindle GO:0005819 IEA
    plasma membrane GO:0005886 ISO
    axoneme GO:0005930 IEA
    axoneme GO:0005930 ISO
    septin ring GO:0005940 IBA
    cell surface GO:0009986 IEA
    cell surface GO:0009986 ISO
    actin cytoskeleton GO:0015629 IEA
    actin cytoskeleton GO:0015629 ISO
    microtubule cytoskeleton GO:0015630 IBA
    midbody GO:0030496 IEA
    septin complex GO:0031105 IBA
    septin complex GO:0031105 IEA
    septin complex GO:0031105 ISO
    cell division site GO:0032153 IBA
    cleavage furrow GO:0032154 IEA
    photoreceptor connecting cilium GO:0032391 IEA
    photoreceptor connecting cilium GO:0032391 ISO
    ciliary transition zone GO:0035869 ISO
    cell projection GO:0042995 ISO
    intercellular bridge GO:0045171 IEA
    intercellular bridge GO:0045171 ISO
    synapse GO:0045202 IEA
    synapse GO:0045202 ISO
    perinuclear region of cytoplasm GO:0048471 IDA
    ciliary membrane GO:0060170 IEA
    ciliary membrane GO:0060170 ISO
    ciliary membrane GO:0060170 ISS
    sperm annulus GO:0097227 IEA
    sperm annulus GO:0097227 ISO
    non-motile cilium GO:0097730 ISO
    presynapse GO:0098793 IDA
 Experiment description of studies that identified Sept2 in exosomes
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Sept2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mib1  
Affinity Capture-MS Rattus norvegicus
2 Park7 117287
Affinity Capture-MS Rattus norvegicus
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