Gene description for CLCN4
Gene name chloride channel, voltage-sensitive 4
Gene symbol CLCN4
Other names/aliases CLC4
ClC-4
ClC-4A
Species Homo sapiens
 Database cross references - CLCN4
ExoCarta ExoCarta_1183
Entrez Gene 1183
HGNC 2022
MIM 302910
UniProt P51793  
 CLCN4 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for CLCN4
Molecular Function
    antiporter activity GO:0015297 IEA
    chloride channel activity GO:0005254 IDA
    ATP binding GO:0005524 IEA
    voltage-gated chloride channel activity GO:0005247 IEA
Biological Process
    ion transmembrane transport GO:0034220 TAS
    chloride transport GO:0006821 IDA
    regulation of anion transmembrane transport GO:1903959 TAS
    transmembrane transport GO:0055085 TAS
    chloride transmembrane transport GO:1902476 IDA
    transport GO:0006810 TAS
Subcellular Localization
    early endosome membrane GO:0031901 IEA
    integral component of membrane GO:0016021 IEA
    endosome membrane GO:0010008 TAS
    late endosome membrane GO:0031902 IEA
 Experiment description of studies that identified CLCN4 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CLCN4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which CLCN4 is involved
PathwayEvidenceSource
Stimuli-sensing channels TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here