Gene description for Arf1
Gene name ADP-ribosylation factor 1
Gene symbol Arf1
Other names/aliases -
Species Mus musculus
 Database cross references - Arf1
ExoCarta ExoCarta_11840
Vesiclepedia VP_11840
Entrez Gene 11840
UniProt P84078  
 Arf1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Arf1
Molecular Function
    magnesium ion binding GO:0000287 IMP
    magnesium ion binding GO:0000287 ISO
    GTPase activity GO:0003924 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IMP
    GTP binding GO:0005525 ISO
    GTP binding GO:0005525 TAS
    GDP binding GO:0019003 ISO
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    phospholipase D activator activity GO:1990583 ISO
Biological Process
    regulation of receptor internalization GO:0002090 ISO
    regulation of receptor internalization GO:0002090 ISS
    intracellular copper ion homeostasis GO:0006878 IEA
    intracellular copper ion homeostasis GO:0006878 ISO
    intracellular protein transport GO:0006886 IBA
    actin filament organization GO:0007015 ISO
    protein transport GO:0015031 TAS
    vesicle-mediated transport GO:0016192 IBA
    vesicle-mediated transport GO:0016192 IDA
    regulation of Arp2/3 complex-mediated actin nucleation GO:0034315 ISO
    regulation of Arp2/3 complex-mediated actin nucleation GO:0034315 ISS
    very-low-density lipoprotein particle assembly GO:0034379 ISO
    positive regulation of endocytosis GO:0045807 ISO
    positive regulation of calcium ion-dependent exocytosis GO:0045956 ISO
    positive regulation of protein secretion GO:0050714 ISO
    Golgi to transport vesicle transport GO:0055108 ISO
    long-term synaptic depression GO:0060292 ISO
    long-term synaptic depression GO:0060292 ISS
    positive regulation of dendritic spine development GO:0060999 ISO
    synaptic vesicle budding GO:0070142 ISO
    dendritic spine organization GO:0097061 ISO
    dendritic spine organization GO:0097061 ISS
    lysosomal membrane organization GO:0097212 ISO
    cellular response to virus GO:0098586 IEA
    cellular response to virus GO:0098586 ISO
    postsynaptic actin cytoskeleton organization GO:0098974 ISO
    positive regulation of sodium ion transmembrane transport GO:1902307 ISO
    positive regulation of late endosome to lysosome transport GO:1902824 ISO
    positive regulation of ER to Golgi vesicle-mediated transport GO:1902953 ISO
    regulation of phospholipid metabolic process GO:1903725 ISO
    mitotic cleavage furrow ingression GO:1990386 IGI
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    Golgi membrane GO:0000139 ISO
    cytoplasm GO:0005737 IBA
    late endosome GO:0005770 ISO
    peroxisomal membrane GO:0005778 ISO
    Golgi apparatus GO:0005794 IDA
    trans-Golgi network GO:0005802 ISO
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    endomembrane system GO:0012505 IDA
    postsynaptic density GO:0014069 IEA
    postsynaptic density GO:0014069 ISO
    sarcomere GO:0030017 IDA
    COPI-coated vesicle GO:0030137 ISO
    cell leading edge GO:0031252 IEA
    cell leading edge GO:0031252 ISO
    protein-containing complex GO:0032991 IMP
    protein-containing complex GO:0032991 ISO
    neuron projection GO:0043005 IEA
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Arf1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Arf1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atxn1  
Affinity Capture-MS Mus musculus
2 PLA2G6 8398
Affinity Capture-Western Homo sapiens
3 Gga3  
Affinity Capture-Western Mus musculus
4 GGA1 26088
Co-crystal Structure Homo sapiens
5 Unk  
Affinity Capture-RNA Mus musculus
6 Fancd2  
Affinity Capture-MS Mus musculus
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