Gene ontology annotations for Atp6v1b2
Experiment description of studies that identified Atp6v1b2 in sEVs
1
Experiment ID
907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
37309723
Organism
Mus musculus
Experiment description
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors
"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name
Proteomics
Publication year
2023
Sample
Colon cancer cells
Sample name
C26
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
2
Experiment ID
210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23260141
Organism
Mus musculus
Experiment description
Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors
"Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name
Cell
Publication year
2012
Sample
Fibroblasts
Sample name
Normal-Fibroblasts (L cells)
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
3
Experiment ID
214
MISEV standards
✔
EM
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23658846
Organism
Mus musculus
Experiment description
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors
"Hassani K, Olivier M."
Journal name
PLoS Negl Trop Dis
Publication year
2013
Sample
Macrophages
Sample name
Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
4
Experiment ID
215
MISEV standards
✔
EM
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23658846
Organism
Mus musculus
Experiment description
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors
"Hassani K, Olivier M."
Journal name
PLoS Negl Trop Dis
Publication year
2013
Sample
Macrophages
Sample name
LPS-treated-Macrophage (J774A.1)
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
5
Experiment ID
216
MISEV standards
✔
EM
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23658846
Organism
Mus musculus
Experiment description
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors
"Hassani K, Olivier M."
Journal name
PLoS Negl Trop Dis
Publication year
2013
Sample
Macrophages
Sample name
Normal-Macrophage (J774A.1)
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
6
Experiment ID
188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19351151
Organism
Mus musculus
Experiment description
Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors
"Lee HS, Jeong J, Lee KJ."
Journal name
J Proteome Res
Publication year
2009
Sample
Pancreatic cells
Sample name
Pancreatic beta cell (NIT-1)
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
7
Experiment ID
908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
37309723
Organism
Mus musculus
Experiment description
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors
"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name
Proteomics
Publication year
2023
Sample
T-cell lymphoma cells
Sample name
EL4
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
Protein-protein interactions for Atp6v1b2
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
Rraga
Co-fractionation
Mus musculus
2
SNCA
6622
Affinity Capture-MS
Homo sapiens
3
Atxn1
Affinity Capture-MS
Mus musculus
4
Kctd13
Affinity Capture-MS
Mus musculus
5
Rragc
Co-fractionation
Mus musculus
6
Tfe3
Affinity Capture-MS
Mus musculus
7
Swap70
Co-fractionation
Mus musculus
8
Atp6v1g2
Co-fractionation
Mus musculus
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Pathways in which Atp6v1b2 is involved