Gene description for Bub3
Gene name budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae)
Gene symbol Bub3
Other names/aliases AU019800
AU021329
AU043350
AW146323
C78067
Species Mus musculus
 Database cross references - Bub3
ExoCarta ExoCarta_12237
Vesiclepedia VP_12237
Entrez Gene 12237
UniProt Q9WVA3  
 Bub3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Bub3
Molecular Function
    ubiquitin binding GO:0043130 IBA
Biological Process
    mitotic spindle assembly checkpoint signaling GO:0007094 IBA
    mitotic spindle assembly checkpoint signaling GO:0007094 NAS
    attachment of spindle microtubules to kinetochore GO:0008608 IEA
    attachment of spindle microtubules to kinetochore GO:0008608 ISO
    protein localization to kinetochore GO:0034501 IEA
    protein localization to kinetochore GO:0034501 ISO
    cell division GO:0051301 IEA
    meiotic cell cycle GO:0051321 IEA
    regulation of chromosome segregation GO:0051983 IDA
Subcellular Localization
    kinetochore GO:0000776 IBA
    kinetochore GO:0000776 IDA
    kinetochore GO:0000776 ISO
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    mitotic checkpoint complex GO:0033597 IBA
    mitotic checkpoint complex GO:0033597 ISO
    bub1-bub3 complex GO:1990298 IBA
 Experiment description of studies that identified Bub3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Bub3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Clip1  
Co-fractionation Mus musculus
2 Cebpa  
Affinity Capture-MS Mus musculus
3 Eed  
Affinity Capture-MS Mus musculus
4 Fxr1 14359
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Bub3 is involved
PathwayEvidenceSource
Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins IEA Reactome
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal IEA Reactome
Amplification of signal from the kinetochores IEA Reactome
APC-Cdc20 mediated degradation of Nek2A IEA Reactome
APC/C-mediated degradation of cell cycle proteins IEA Reactome
APC/C:Cdc20 mediated degradation of mitotic proteins IEA Reactome
APC:Cdc20 mediated degradation of cell cycle proteins prior to satisfation of the cell cycle checkpoint IEA Reactome
Cdc20:Phospho-APC/C mediated degradation of Cyclin A IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
EML4 and NUDC in mitotic spindle formation IEA Reactome
Inactivation of APC/C via direct inhibition of the APC/C complex IEA Reactome
Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components IEA Reactome
M Phase IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase IEA Reactome
Mitotic Spindle Checkpoint IEA Reactome
Regulation of APC/C activators between G1/S and early anaphase IEA Reactome
Regulation of mitotic cell cycle IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here