Gene description for Serpinh1
Gene name serine (or cysteine) peptidase inhibitor, clade H, member 1
Gene symbol Serpinh1
Other names/aliases BERF-1
Cbp1
Cbp2
Hsp47
J6
Serpinh2
gp46
Species Mus musculus
 Database cross references - Serpinh1
ExoCarta ExoCarta_12406
Vesiclepedia VP_12406
Entrez Gene 12406
UniProt P19324  
 Serpinh1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Serpinh1
Molecular Function
    serine-type endopeptidase inhibitor activity GO:0004867 IBA
    serine-type endopeptidase inhibitor activity GO:0004867 IEA
    protein binding GO:0005515 IPI
    collagen binding GO:0005518 IEA
    unfolded protein binding GO:0051082 IDA
Biological Process
    chondrocyte development involved in endochondral bone morphogenesis GO:0003433 IMP
    negative regulation of endopeptidase activity GO:0010951 IBA
    collagen fibril organization GO:0030199 IBA
    collagen fibril organization GO:0030199 IMP
    collagen biosynthetic process GO:0032964 IMP
    protein maturation GO:0051604 IMP
Subcellular Localization
    extracellular space GO:0005615 IEA
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum lumen GO:0005788 IEA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IEA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 ISO
    membrane raft GO:0045121 IEA
    membrane raft GO:0045121 ISO
    collagen-containing extracellular matrix GO:0062023 HDA
 Experiment description of studies that identified Serpinh1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Serpinh1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mettl21e  
Affinity Capture-MS Mus musculus
2 Col5a1 12831
Co-fractionation Mus musculus
3 Mmp2  
Co-fractionation Mus musculus
4 Kcna3  
Affinity Capture-MS Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Lipc  
Affinity Capture-MS Mus musculus
7 Col4a2 12827
Co-fractionation Mus musculus
8 Pou5f1  
Affinity Capture-MS Mus musculus
9 Col5a2  
Co-fractionation Mus musculus
10 Fancd2  
Affinity Capture-MS Mus musculus
11 HACE1 57531
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Serpinh1 is involved
PathwayEvidenceSource
Collagen biosynthesis and modifying enzymes IEA Reactome
Collagen formation IEA Reactome
Extracellular matrix organization IEA Reactome





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